Matrix metalloproteinases activity during the evolution of hypoxic-ischemic brain damage in the immature rat. The effect of 1-methylnicotinamide (MNA)

• Autorzy: Dragun P. , Makarewicz D. , Wojcik L. , Ziemka-Nalecz M. , Slomka M. , Zalewska T.
• Języki publikacji: EN

Abstrakty

EN

Matrix metalloproteinases (MMPs) are a family of proteolytic enzymes that degrade the extracellular matrix and carry out key functions during brain development. Apart from a physiological role, excessive activation of MMPs in brain tissue has been postulated to represent a pathway for cell death arising from ischemia. To evaluate the possible involvement of MMPs in the perinatal brain asphyxia, we exposed 7-day-old rats to hypoxia-ischemia (HI). Unilateral HI was administered by ligation of the common carotid artery followed by hypoxia (7.4% O2/92.6% N2) for 65 minutes. This insult is known to produce brain damage confined to the cerebral hemisphere ipsilateral to the arterial occlusion in > 90% of animals. HI resulted in a significant elevation of MMP-2 and MMP-9 activity in the ipsilateral forebrain. The maximum activation was found at 48 hours and 7-14 days after the insult. These results suggest that early and late induction of MMPs may play a role in neuronal death as well as in repair processes. The treatment of animals subjected to HI with 1-methylnicotinamide (MNA), the anti-inflammatory agent, led to the inhibition of MMP-9 in an acute phase of ischemic damage and to the activation of MMP-2 in the later stages after injury. The timing of MMPs modulation by MNA may indicate its possible therapeutic implications.

Słowa kluczowe

EN

antiinflammatory agent; immature rat; proteolytic enzyme; matrix metalloproteinase; brain damage; rat; ischaemia; 1-methylnicotinamide

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2008
• Tom: 59
• Numer: 3
• Strony: 441-455
• Opis fizyczny: p.441-455,fig.,ref.

Twórcy

autor

Dragun P.

• Polish Academy of Sciences, 5 Pawinskiego Str., 02-106 Warsaw, Poland

autor

Makarewicz D.

autor

Wojcik L.

autor

Ziemka-Nalecz M.

autor

Slomka M.

autor

Zalewska T.