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Abstrakty
Our objective was the assessment of algal medium harmfulness for in vitro fibroblasts. The algal medium was from Chlorella cultures (Beijerinck 1890) grown in the presence of benzene, which was added before the inoculation of culture. The medium contained, besides the benzene residues, its various metabolites formed during culture, among them phenol and catechol. Its addition to fibroblast cultures resulted in a decrease in their growth intensity and protein content in fibroblasts as well as an increase in DNA content and 14C-thymidine incorporation intensity to fibroblasts. The latter effect was probably connected with the inhibition of cell-division and DNA damage reparations. The obtained results indicate that Chlorella algae, besides other hydrobionts, take part in the forming of the benzene-like pollution toxicity in water habitat.
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Tom
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Opis fizyczny
p.27-30
Twórcy
autor
- Silesian University School, Medykow 18, 40-752 Katowice, Poland
autor
autor
Bibliografia
- CERNIGLIA C.E., Aromatic hydrocarbons: Metabolism by bacteria, fungi and algae. Hodgson E., Bend J.R., Philpot R.M. (eds). Reviews in Biochemical Toxicology 3. Elsevier, North-Holland, New York, Amsterdam, Oxford, pp 321-361,1983.
- WARDAS W., The interaction of egzogenous hydrocarbons with Chlorella algae in aqueous medium. Scientific Papers of the Institute of Environment Protection Engineering of Wroclaw Technical University, No 53, Monographs No 22, Wrocław 1983. In Polish.
- GUNKEL W., GASMANN G., Oil, oil dispertants and related substances in the marine environment. Helgol. Meeresunters 33, 164-169,1980.
- WĘGLARZ L., DRÓŻDŻ M., WARDAS M., KULA B„ PAWLACZYK-SZPILOWA M., Usefulness of fibroblast culture for testing of cattle tissues polluted with heavy metals. Environ. Res. 51, 163-169,1990.
- WARDAS W., MAJNUSZ U., MAKOWSKI A., Benzene metabolism in Chlorella cells. Abstr. of 24-th Congress of Polish Biochemical Society, Poznań, pp 43,1988. In Polish.
- WARDAS W., MAJNUSZ U., MAKOWSKI A., SOCHA¬CKA J., PIERON G., BOCHEŃSKA T., Benzene, toluene and m-xylene metabolites in laboratory cultures of Chlorella algae. Report No 3, Polish Academy of Science, Institute of Oceanology, Sopot, Poland In Polish.
- WARDAS W., MAJNUSZ U, SKOREK R., The influence of benzene, phenol and catechol on Chlorella algae growth. Abstr. of 24th Congress of Polish Biochemical Society, Poznań, pp 44,1988. In Polish.
- FOTT B., Algenkunde. VEB Gustav Fisher Verlag, Jena, 1971.
- BORNS E., BOHM H., SCHULZE D., Untersuchungen zum Einfluss Vershiedener Nahrlösungskombinationen auf das Wachstum synchroner Kulturen von Chlorella vulgaris. Wissenschaftliche Hefte der Pädagogischen Hochschule ’Wolfgang Ratke’, Köthen, pp 55-62,1973.
- PAUL J., Cell and tissue culture. Livingstone, Edinburgh London, 1980.
- PHILIPS H.J., Dye exclusion test for cell viability. Kruse P.F., Patterson M.K., Jr (eds). Tissue culture methods and application. Academic Press, New York, pp 406-408, 1973.
- SORGER T., GERMINARIO R.J., A direct solubilization procedure for the determination of DNA and protein in cultured fibroblast monolayers. Anal. Biochem. 131, 254-256,1983.
- LABARCA C., PAIGEN K., A simple, rapid and sensitive DNA assay procedure. Anal. Biochem. 102, 344-352,1980.
- WARDAS W., WARDAS M., MAZUREK U., WASZKIE¬WICZ E., MAJNUSZ U., Toxicity of certain hydrocarbons against Chlorella algae in laboratory cultures. Roczniki PZH, XXXVIII (No 6), 523-529, 1987. In Polish.
- WARDAS M., WARDAS W., MAZUREK U., LECHO- TYCKA E., The effect of some organie solvents on the growth of Chlorella algae, Strain 366. Oceanologia 17, 21-27,1983.
- SMART R.C., ZANNONI V.G., DT-diaphorase and peroxidase influence the covalent binding of the metabolites of phenol, the major metabolite of benzene. Mol. Pharma¬col, 26, 105-111, 1984.
- KINOSHITA T., SANTELA R., PULKRABEK P., JEF¬FREY A.M., Benzene oxide: genetic toxicity. Mutation Research, 91, 99-102, 1981.
- JOHANSON J., INGELMAN-SUNDBERG M., Hydroxyl radical-mediated, cytochrome P-450 - dependent metabolic activation of benzene in microsomes and reconstituted enzyme systems from rabbit liver. J. Biol. Chem., 258 (12), 7311-7316, 1983.
Typ dokumentu
Bibliografia
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