Wybrane cechy warunkujace chorobotworczosc Staphylococcus haemolyticus

• Autorzy: Bartoszewicz M. , Nowicka J. , Przondo-Mordarska A.
• Języki publikacji: PL

Abstrakty

PL

Badania przeprowadzono na 44 szczepach Staphylococcus haemolyticus pochodzących z próbek materiału klinicznego pobranych od pacjentów hospitalizowanych na Oddziale Chirurgii. Analizowano następujące czynniki warunkujące chorobotwórczość szczepów S. haemolyticus: wytwarzanie śluzu, adhezję do biomateriałów, wrażliwość na antybiotyki oraz profil białek powierzchniowych.

EN

The study have been done on S. haemolyticus strains isolated from patients hospitalized an Surgical Unit. Aim of the study was to determine pathogenic traits of S. haemolyticus: slime producing, adhesion to biomateriale, antibiotics susceptibility and the profiles of surface proteins. Among 44 S. haemolyticus strains, in the test-tube method, there have been 38% labeled as slime producing and 62% as non-producing. In the plate method at 48% slime production was noticed, while 52% strains did not produce slime. It is quite significant that all CNS strains which have an ability to produce mucus, that was proved by means of two methods (test-tube and plate), show a high level of TTC's reduction to formazan. The analysis of resistance to antibiotics in relation to slime production demonstrated more frequent antibiotic resistance of the slime-producing strains.

Słowa kluczowe

PL

sluz; gronkowce; szczepy bakteryjne; adhezja; chorobotworczosc; zakazenia szpitalne; Staphylococcus haemolyticus

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2003
• Tom: 55
• Numer: 3
• Strony: 225-229
• Opis fizyczny: s.225-229,tab.,rys.,bliogr.

Twórcy

autor

Bartoszewicz M.

• Akademia Medyczna, Wroclaw

autor

Nowicka J.

autor

Przondo-Mordarska A.

Bibliografia

• 1. Carballo J, Ferreiros CM, Criado MT. Importance of experimental design in the evaluation of the influence of proteins in bacterial adherence to polymers. Med Microbiol Immunol 1991; 180:149-55.
• 2. Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime-producing strains of Staphylococcus epidermidis to smooth surfaces. Infection and Immunity 1982; 37: 318-26.
• 3. Eliopoulos GM. Vancomycin resistant enterococci. Mechanism and clinical relevance. Infect Dis Clin No Am 1997; 11: 851-65.
• 4. Fleeer A, Timmerman CP, Besnier JM, i inni. J. Surface proteins of coagulase-negative staphylococci: their role in adherence to biomateriale and in opsonization. J Biomaterials Applications 1990; 5: 154-65.
• 5. Huebner J, Goldmann AD. Coagulase-negative staphylococci: role as pathogens. Annu Rev Med 1999; 50: 223-36.
• 6. Hussain M, Wilcox MH, White PJ. The slime of coagulase-negative staphylococci: Biochemistry and relation to adherence. FEMS Microbiol Reviews 1993; 104: 191-208.
• 7. Ishak MA, Groschel DH. Assosation of slime with pathogenity of coagulase-negative staphylococci causing nosocomial septicemia. J Clin Microbiol 1985; 22: 1025-9.
• 8. Jones W J, Scott RJD, Morgan J, Pether JVS. A study of coagulase-negative with reference to slime production, adherence, antibiotic resistance and clinical significance. Journal of Hospital Infection 1992; 22: 217-27.
• 9. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacetriophage T4. Nature (London). 1970, 227, 680-5.
• 10. Ludwicka A, Jansen В, Wadstrom T, Pulverer G. Attachment of staphylococci to various synthetic polymers. Zbl Bakt Hyg A 1984; 256: 479-89.
• 11. Różalska В, Sadowska В, Więckowska M, Rudnicka W. Wykrywanie biofilmu bakteryjnego na biomateriałach medycznych. Med. Dośw Mikrobiol 1998; 50: 115-22.
• 12. Tabe Y, Nakamura A, Igari J. Glycopeptide susceptibility profiles of nosocomial multiresistant Staphylococcus haemolyticus isolates. J Infect Chemother 2001; 7: 142-7.
• 13. Weistein MP, Mirrett S, Pelt L, i inni. Clinical importance of identyfying coagulase- negative staphylococci isolated fromblood cultures: evaluation of MicroScan rapid and dried overnight Gram - positive panels versus a conventional reference method. J Clin Microbiol 1998; 36: 2089-92.