Antitumoral effect of IL-12 gene transfected via liposomes into B16F0 cells

• Autorzy: Speroni L. , Gasparri J. , de los A.Bustuoabad V. , Chiaramoni N.S. , Smagur A. , Szala S. , Taira M.C. , del V.Alonso S.
• Języki publikacji: EN

Abstrakty

EN

 Murine melanoma B16F0 cells were transfected with SA:DPPC:DOPE (2:1:1 molar ratio) liposomes associated with a plasmid encoding murine IL-12. Stearylamine, a cationic lipid, showed a greater transfection efficiency compared to DOTAP-containing liposomes. The lipid:DNA ratio was 2:1 (w/w). Control groups were mock transfected or transfected with an empty plasmid (pNeo). pNeo or IL-12 transfected cells and controls were inoculated intradermically into the dorsal region of the foot or the lateral flank of C57BL6 mice. Results showed that IL-12 expression had a marked effect on in vivo growth of B16 melanoma tumors developed in both anatomic sites, significantly retarding their growth and prolonging host survival.

Słowa kluczowe

PL

interleukin 12; murine melanoma; liposome; cationic lipid; melanoma; immunity; stearylamine; cell viability; tumour growth; mice; mouse; survival; cell transfection; plasmid

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2009
• Tom: 56
• Numer: 2
• Strony: 249-253
• Opis fizyczny: p.249-253,fig.,ref.

Twórcy

autor

Speroni L.

• Universidad Nacional de Quilmes, Buenos Aires, Argentina

autor

Gasparri J.

autor

de los A.Bustuoabad V.

autor

Chiaramoni N.S.

autor

Smagur A.

autor

Szala S.

autor

Taira M.C.

autor

del V.Alonso S.

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