Roznicowanie szczepow Campylobacter jejuni metoda analizy polimorfizmu przypadkowo amplifikowanego DNA

• Autorzy: Wieczorek K , Osek J.

Warianty tytułu

EN

Differentiation of Campylobacter jejuni isolates by random amplified polymorphic DNA method

• Języki publikacji: PL

Abstrakty

EN

Campylobacter, especially C. jejuni, is now recognized as a major cause of bacterial enteritis in people in most developed countries. The diversity within C. jejuni species may be described by both phenotypic and genotypic methods. In recent years, molecular methods, such as the restriction of fragment length polymorphism (RFLP), fla typing or random amplified polymorphic DNA (RAPD), have been proposed as alternative tests to phenotypic methods for typing of Campylobacter. This study was carried out to investigate the genetic variation among isolates C. jejuni by RAPD-PCR. The analysis of 19 isolates of C. jejuni performed with two primers (1254 and OPA-11) generated multiple amplification products, with sizes between 300 and 2000 bp. The UPGMA analysis of the fingerprints classified these strains to 10 clonal groups. The discriminatory index D=0.73 indicates a high differentiation ability of the method. Such data are valuable in epidemiological studies and for investigating the distribution of genotypes in different environments.

Słowa kluczowe

PL

szczepy bakteryjne; odchody zwierzece; metoda RAPD-PCR; izolacja; Campylobacter jejuni; bakterie; roznicowanie; metody; tuszki drobiowe

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 06
• Strony: 663-664
• Opis fizyczny: s.663-664,rys.,bibliogr.

Twórcy

autor

Wieczorek K

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

autor

Osek J.

Bibliografia

• 1.Akopyanz N., Bukanov N. O., Westblom T. U., Kresovich S., Berg D. E.: DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res. 1992, 20, 5137-5142.
• 2.Denis M., Soumet C., Rivoal K., Ermel G., Blivet D., Salvat G., Colin P.: Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli. Lett. Appl. Microbiol. 1999, 29, 406-410.
• 3.Ertas H. B., Cetinkaya B., Muz A., Ongor H.: Genotyping of broiler-originated Campylobacter jejuni and Campylobacter coli isolates using fla typing and random amplified polymorphic DNA methods. Int. J. Food Microbiol. 2004, 94, 203-209.
• 4.Hernandez J., Fayos A., Ferrus M. A., Owen R. J.: Random amplified polymorphic DNA fingerprinting of Campylobacter jejuni and C. coli isolated from human faeces, seawater and poultry products. Res. Microbiol. 1995, 146, 685-696.
• 5.Hilton A. C., Mortiboy D., Banks J. G., Penn C. W.: RAPD analysis of environmental, food and clinical isolates of Campylobacter spp. FEMS Immunol. Med. Microbiol. 1997, 18, 119-124.
• 6.Hunter P. R., Gaston M. A.: Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. J. Clin. Microbiol. 1988, 26, 2465-2466.
• 7.Madden R. H., Moran L., Scates P.: Frequency of occurrence of Campylobacter spp. in red meats and poultry in Northern Ireland and their subsequent subtyping using polymerase chain reaction-restriction fragment length polymorphism and the random amplified polymorphic DNA method. J. Appl. Microbiol. 1998, 84, 703-708.
• 8.Misawa N., Shinohara S., Satoh H., Itoh H., Shinohara K., Shimomura K., Kondo F., Itoh K.: Isolation of Campylobacter species from zoo animals and polymerase chain reaction-based random amplified polymorphism DNA analysis. Vet. Microbiol. 2000, 71, 59-68.
• 9.Wassenaar T. M., Newell D. G.: Genotyping of Campylobacter spp. Appl. Environ. Microbiol. 2000, 66, 1-9.