Substrate specifity and inhibitors of polyphenol oxidase in aspect of darkening of fresh and frozen mushrooms [Agaricus bisporus [Lange] Sing.]

• Autorzy: Czapski J

Warianty tytułu

PL

Specyficznosc substratowa i inhibitory oksydazy polifenolowej w aspekcie ciemnienia swiezych i mrozonych pieczarek [Agaricus bisporus Lange] Sing.]

• Języki publikacji: EN

Abstrakty

EN

Activity of mushroom polyphenol oxidase (PPO) toward 6 substrates and inhibitory effect of cysteine, 2-mercaptoethanol, benzoic acid and sodium metabisulphite were determined. The o-diphenols which appeared to be the best substrates were: catechin, DOPA (L-3,4-dihydroxyphenylalanine) and chlorogenic acid. Affinity of PPO crude preparation substrates to enzyme, expressed as inverse value of Michaelis constant was lower then affinity of catechol. Inhibitory effect depended on specifity of inhibitors and their concentration. Electrophoretic patterns of PPO of mushrooms reveals slow and fast moving 4 isoforms when DOPA was used as a substrate, 2 bands for catechin and chlorogenic acid while only one band showed activity toward tyrosine and p-cresol.

PL

Badano aktywność oksydazy polifenolowej pieczarek wobec 6 substratów oraz hamowanie aktywności enzymu przez cysteinę, 2-merkaptoetanol, kwas benzoesowy i polisiarczyn sodu. Najbardziej efektywnymi substratami byly: katechina, DOPA (L-3,4-dihydroksyfenyloalanina) i kwas chlorogenowy. Powinowactwo enzymu do endogennych substratów wyrażone odwrotności.) stałej Michaelisa (Km) bylo niższe niż powinowactwo do katecholu. Efekt hamowania aktywności zależał od specyfiki i stężenia inhibitora w roztworze. Elektroforetyczne rozdzielenie izozymów oksydazy polifenolowej wykazało obecność 4 izozymów wobec DOPA, dwóch wobec katechiny i kwasu chlorogenowego oraz jednego wobec L-tyrozyny i p-krezolu.

Słowa kluczowe

PL

aktywnosc enzymatyczna; inhibitory; pieczarki; specyficznosc substratowa; ciemnienie grzybow; oksydaza polifenolowa

EN

enzyme activity; inhibitor; mushroom; substrate specificity; mushroom darkening; polyphenol oxidase

• Wydawca: -
• Czasopismo: Acta Agrobotanica
• Rocznik: 1994
• Tom: 47
• Numer: 1
• Strony: 103-110
• Opis fizyczny: s.103-110,rys.,tab.,bibliogr.

Twórcy

autor

Czapski J

• Instytut Warzywnictwa, 96-100 Skierniewice, Konstytucji 3 Maja 1/3

Bibliografia

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