Wplyw zwiazkow alkilujacych na indukcje zaberrowanych krypt w jelicie grubym szczurow rasy Wistar i myszy rasy Swiss

• Autorzy: Czeczot H. , Tudek B.
• Języki publikacji: PL

Abstrakty

PL

Badano wpływ związków alkilujących (DMH, MNU, MNNG, MMS) na indukcję zaberrowanych krypt w jelicie grubym samic szczurów rasy Wistar i myszy rasy Swiss w zależności od dawki, drogi podawania i wieku.

EN

The aim of this work was to study the effect of 4 alkylating agents: 1,2-dimethylhydrazine (DMH), N-nitrosomethylurea (MNU); N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and methyl-methane sulphonate (MMS) on the induction of aberrant crypts (AC) in the colon of female Wistar rats and Swiss mice. It was shown that the standard colon carcinogens (DMH and MNU), when administered intragastrically (per os) to the rats and mice, induced aberrant crypts in the dose-dependent manner. It was also found that 9—10 week rats were more sensitive to abberant crypts induction than the younger 3—4 week animals. The aberrant crypts induced by DMH in the colons of 3—4 week female Wistar rats and 3-week female Swiss mice were more frequent in the middle colon and rectum. MNNG and MMS, administrered in our experiment per os and per rectum only to the 3—4 week-old female rats, also induced AC in the colon. The aberrant crypts were evidently more frequent in the animals receiving MNNG per rectum. Among the studied chemicals, MMS induced relatively few aberrant crypts in the colon of the female rats, and only at higher concentrations. The results of our study confirm the hypothesis that aberrant crypts are biomarkers of colonic preneoplastic lesions and demonstrate that the method is useful as a short-term assay for carinogenicity; it may be also used for studying the carcinogenic process in the colon.

Słowa kluczowe

PL

metylonitrozomocznik; szczury; metoda Aberrant Crypts Assay; jelito grube; zwierzeta laboratoryjne; N-metylo-N-nitro-N-nitrozoguanidyna; aberracje; fizjologia czlowieka; 1,2-dimetylohydrazyna; wiek; metylometanosulfonian; zwiazki alkalizujace

EN

rat; large intestine; laboratory animal; N-methyl-N'-nitro-N-nitrosoguanidine; aberration; human physiology; 1,2-dimethylhydrazine; animal age; alkylating compound

• Wydawca: -
• Czasopismo: Bromatologia i Chemia Toksykologiczna
• Rocznik: 1995
• Tom: 28
• Numer: 1
• Strony: 79-86
• Opis fizyczny: s.79-86,rys.,wykr.

Twórcy

autor

Czeczot H.

• Akademia Medyczna, Warszawa

autor

Tudek B.

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