Prophylactic potential of montelukast against mild colitis induced by dextran sulphate sodium in rats

• Autorzy: Holma R. , Salmenpera P. , Virtanen I. , Vapaatalo H. , Korpela R.
• Języki publikacji: EN

Abstrakty

EN

Cysteinyl leukotrienes play a part in inflammatory processes such as inflammatory bowel diseases. The present study aimed to evaluate the effects of the cys-LT-1 receptor antagonist montelukast on a mild colitis model in rats. Colitis was induced by administrating 4% dextran sulphate sodium (DSS, MW 45 000) in drinking water for 9 days. Montelukast (10 mg/kg/day) or vehicle was given by gastric gavage once daily simultaneously with DSS administration. A healthy control group receiving water as drinking fluid and vehicle by gastric gavage was included. Body weight loss, consistency of faeces (loose/diarrhoea) and occult blood in the faeces/ gross bleeding were assessed on days 6 - 9. After sacrifice, the following were assessed: colonic histology, the expression of inducible nitric oxide synthase, macrophage/monocyte marker ED1, cyclooxygenase-1 and cyclooxygenase-2, as well as the production of leukotriene B4 and E4, prostaglandin E2, its metabolite bicyclic-prostaglandin E2 and thromboxane B2 in the colonic tissue incubation in vitro. Rats receiving DSS exhibited bloody diarrhoea from day 6 onwards. Montelukast significantly reduced the occult blood in the faeces/ gross bleeding, maintained normal body weight gain and tended to decrease the ratio of leukotriene B4/ prostaglandin E2 production in the colon in vitro. The results indicate that montelukast has some potential to ameliorate mild experimental colitis induced by DSS.

Słowa kluczowe

EN

inflammatory bowel disease; cysteinyl leukotriene; inflammatory process; leukotriene B4; colitis; prostaglandin E2; dextran sulphate sodium

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2007
• Tom: 58
• Numer: 3
• Opis fizyczny: p.455-467,fig.,ref.

Twórcy

autor

Holma R.

• University of Helsinki, P.O.Box 63, FIN-00014, Finland

autor

Salmenpera P.

autor

Virtanen I.

autor

Vapaatalo H.

autor

Korpela R.