Molecular identification of hairtail species [Pisces: Trichiuridae] based on PCR-RFLP analysis of the mitochondrial 16S rRNA gene

• Autorzy: Chakraborty A , Aranishi F. , Iwatsuki Y.
• Języki publikacji: EN

Abstrakty

EN

A rapid PCR-RFLP analysis was designed to identify 3 closely related species of hairtails: Trichiurus lepturus, T.japonicus, and Trichiurus sp. 2, basing on partial sequence data (600 bp) of the mitochondrial DNA encoding the 16S ribosomal RNA (16S rRNA) gene. Restriction digestion analysis of the unpurified PCR products of these 3 species, using EcoRI and VspI endonucleases, generated reproducible species-specific restriction patterns showing 2 fragments (250 bp and 350 bp) for T. lepturus in EcoRI digestion and 2 fragments (196 bp and 404 bp) for T. japonicus in VspI digestion, whereas no cleavage was observed for Trichiurus sp. 2 in both EcoRI and VspI digestions. The PCR-RFLP technique developed in this study proved to be a rapid, reliable and simple method that enables easy and accurate identification of these 3 closely related species of the genus Trichiurus.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2005
• Tom: 46
• Numer: 4
• Strony: 381-385
• Opis fizyczny: p.381-385,fig.,ref.

Twórcy

autor

Chakraborty A

• Miyazaki University, 1-1 Gakuen-kibanadai-nishi, Miyazaki 889-2192, Japan

autor

Aranishi F.

autor

Iwatsuki Y.

Bibliografia

• Aranishi F, Okimoto T, Izumi S, 2005a. Identification of gadoid species (Pieces, Gadidae) by PCR-RFLP analysis. J Appl Genet 46: 69-73.
• Aranishi F, Okimoto T, Okhubo M, Izumi S, 2005b. Molecular identification of commercial spicy Pollack roe products by PCR-RFLP analysis. J Food Sci 70: 235-238.
• Bourdy P, Heurtebise S, Lapegue S, 2003. Mitochondrial and nuclear DNA sequence variation of presumed Crassostrea gigas and Crassostrea cingulcita specimens: A new oyster species in Hong Kong? Aquaculture 228: 15-25.
• Chakraborty A, Aranishi F, Iwatsuki Y, 2006. Genetic differences among three species of the genus Trichiurus (Perciformes: Trichiuridae) based on mitochondrial DNA analysis. Ichthyol Res (in press).
• Karaiskou N, Apostolidis AP, Triantafyllidis A, Kouvatsi A, Triantaphyllidis C, 2003. Genetic identification and phylogeny of three species of the genus Trachurus based on mitochondrial DNA analysis. Mar Biotechnol 5: 493-504.
• Mabuchi K, Okuda N, Kokita T, Nishida M, 2003. Genetic comparison of two color-morphs of Apogon properuptus from southern Japan. Ichthyol Res 50: 293-296.
• Miya M, Nishida M, 1996. Molecular phylogenetic perspective on the evolution of the deep-sea fish genus Cyclothone (Stomiiformes: Gonostoma- tidae). Ichthyol Res 43: 375-398.
• Mochijuki K, 1997. Pictorial encyclopedia of fish and shellfish. Kashiwa-Shobo Publ: 247-248.
• Nakamura I, Parin NV, 1993. FAO Species Catalogue. Vol. 15. Snake mackerels and cutlassfishes of the world (families Gempylidae and Trichiuridae). An annotated and illustrated catalogue of the snake mackerels, snoeks, escolars, gemfishes, sackfishes, domine, oilfish, cutlassfishes, scabbardfishes, hairtails and frostfishes known to date. FAO, Rome.
• Nakabo T, 2002. Family Trichiuridae. In: Nakabo T, ed. Fishes of Japan with pictorial keys to the species. Tokai University Press: 1749-1750.
• Palumbi SR, 1996. Nucleic acid II: the polymerase chain reaction. In: Hillis DM, Moritz C, Mable BK, eds. Molecular Systematics. Sunderland, Sinauer Associates.
• Quinteiro J, Sotelo CG, Rehbein H, Pryde SE, Perez-Martin RI, Rey-Mendez M, Mackie IM, 1998. Use of mtDNA direct polymerase chain reaction (PCR) sequencing and PCR-restriction fragment length polymorphism methodologies in species identification of canned tuna. J Agrie Food Chem 46: 1662-1669.
• Sebastio P, Zanelli P, Neri TM, 2001. Identification of anchovy (Engraulis encrasicholus L.) and gilt sardine (Sardinella aurita) by polymerase chain reaction, sequence of their mitochondrial cytochrome b gene and restriction analysis of polymerase chain reaction products in semipreserves. J Agrie Food Chem 49: 1194-1199.
• Tucker DW, 1956. Studies on trichiuroid fishes. A preliminary revision of the family Trichiuridae. Bull Brit Mus Nat Hist Zool 4: 73-130.