Effects of Cu2plus, cytokinins and jasmonate on content of two flavonols identified in zucchini cotyledons

• Autorzy: Stoynova-Bakalova E , Nikolova M. , Maksymiec W.
• Języki publikacji: EN

Abstrakty

EN

This study examined the effects of cytokinins and methyl jasmonate (JAMe) alone and in combination on the growth and flavonol content of zucchini cotyledons cultured in the presence or absence of Cu2+. During greening of cotyledons in intensive diurnal illumination, rutin was identified as the main flavonol compound. Its accumulation was greatly stimulated by phenylurea cytokinin (4PU-30) but reduced by Cu2+. Stimulation by benzylaminopurine (BA) was less. JAMe showed an inhibitory effect, alone and with simultaneous addition of Cu2+ or cytokinins. In moderate excess (100 μM and 250 μM CuSO4), Cu2+ enhanced stimulation by 4PU-30 of rutin accumulation; at a higher concentration or with other substances it decreased the rutin level. The other flavonol detected, kaempferol-3-rhamnoside, increased after JAMe or 4PU-30 were added; in the other cases it decreased. The data obtained indicate that cytokinins can increase rutin content in developing Cucurbita cotyledons. Rutin content usually decreased under stress induced by excess Cu2+, but Cu2+ in moderate excess had a stimulating effect in the presence of higher phenylurea cytokinin levels

Słowa kluczowe

EN

copper; Cucurbita pepo; cytokinin; jasmonate; flavonol content; zucchini; cotyledon; flavonoids; growth; heavy metal; rutin

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2009
• Tom: 51
• Numer: 2
• Strony: 77-83
• Opis fizyczny: p.77-83,fig.,ref.

Twórcy

autor

Stoynova-Bakalova E

• Bulgarian Academy of Sciences, Acad.G.Bonchev Str., Bl.21, 1113 Sofia, Bulgaria

autor

Nikolova M.

autor

Maksymiec W.

Bibliografia

• Abreu IN, Porto ALM, Marsaioli AJ, and Mazzafera P. 2004. Distribution of bioactive substances from Hypericum brasiliense during plant growth. Plant Science 167: 949-954.
• Ali RM, and Abbas HM. 2003. Response of salt stressed barley seedlings to phenylurea. Plant, Soil and Environment 49: 158-62.
• Angelova Y, Petkova S, Zozikova E, Kotseva E, and Iliev L. 2001. Effects of kinetin and 4PU-30 on the growth and the content of polyphenols in tobacco callus tissue. Bulgarian Journal of Plant Physiology 27: 36-42.
• Awad MA, Wagenmakers PS, and de Jager A. 2001. Effects of light on flavonoid and chlorogenic acid levels in the skin of 'Jonagold' apples. Scientia Horticulturae 88: 289-298.
• Belhadj A, Telef N, Saigne C, Cluzet S, Barrieu F, Hamdi S, and Mérillon J-M. 2008. Effect of methyl jasmonate in combination with carbohydrates on gene expression of PR proteins, stilbene and anthocyanin accumulation in grapevine cell cultures. Plant Physiology and Biochemistry 46: 493-499.
• Cooper-Driver G, and Bhattacharya M. 1998. Role of phenolics in plant evolution. Phytochemistry 49: 1165-1174.
• Curir P, VanSumere CF, Termini A, Barthe P, Marchesin A, and Dolci M. 1990. Flavonoid accumulation is correlated with adventitious roots formation in Eucalyptus gunnii hook micropropagated through axillary bud stimulation. Plant Physiology 92: 1148-1153.
• Dedio W, and Clark KW. 2006. Influence of cytokinins on isoflavone and anthocyanin synthesis in red clover seedlings. Pesticide Science 2: 65-68.
• Del Rho JA, Marhn FR, Fuster MD, Benavente O, and Ortuco A. 1998. Regulation of flavonoid expression in Citrus and Hyssopus officinalis plants. 2nd international Electronic Conference on Synthetic Organic Chemistry (ECSOC-2), September 1-30, 1998 http://www.mdpi.org/ecsoc/.
• Farag M, Huhman DV, Dixon R, and Sumner LW. 2008. Metabolomics reveals novel pathways and differential mechanistic and elicitor-specific responses in phenyl- propanoids and isoflavonoid biosynthesis in Medicago truncatula cell cultures. Plant Physiology 146: 387-402.
• Goossens JFV, and Vendrig JC. 1982. Effects of abscisic acid, cytokinins, and light on isoflavonoid phytoalexin accumulation in Phaseolus vulgaris L. Planta 154: 441-446.
• Hendrawati O, Yao Q, Kim HK, Linthorst HJM, Erkelens C, Lefeber AWM, Choi YH, and Verpoorte R. 2006. Metabolic differentiation of Arabidopsis treated with methyl jasmonate using nuclear magnetic resonance spectroscopy. Plant Science 170: 1118-1124.
• Horbowicz M, Grzesiuk A, Dębski H, Koczkodan D, and Saniewski M. 2008. Methyl jasmonate inhibits anthocyanin synthesis in seedlings of common buckwheat (Fragophyrum esculentum Moench). Acta Biologica Cracoviensia Series Botanica 50/2: 71-78.
• Imperato F. 1979. Flavonoids from pollens and stigmas of male and female flowers of four species of the genus Cucurbita. Cellular and Molecular Life Sciences 35: 13-14.
• Ishihara A, Ogura Y, Tebayashi S-I, and Iwamura H. 2002. Jasmonate-induced changes in flavonoids metabolism in barley (Hordeum vulgare) leaves. Bioscience Biotechnology and Biochemistry 66: 2176-2182.
• Izaguirre MM, Mazza CA, Svato SA, Baldwin IT, and Ballare CL. 2007. Solar ultraviolet-B radiation and insect herbivory trigger partially overlapping phenolic responses in Nicotiana attenuata and Nicotiana longiflora. Annals of Botany (London) 99: 103-109.
• Jeoman MM, Miedzybrodzka MB, Lindsey K, and McLauchlan WR. 1980. The synthetic potential of cultured plant cell. in: Sala F, Parisi B, Cella R, and Cifferi O [eds], Plant Cell Culture: Results and Perspectives, 327-343. Elsevier North Holland, Amsterdam.
• Maksymiec W, Wianowska D, Dawidowicz AL, Radkiewicz S, Mardarowicz M, and Krupa Z. 2005. The level of jasmonic acid in Arabidopsis thaliana and Phaseolus coc- cineus plants under heavy metal stress. Journal of Plant Physiology 162: 1338-1346.
• Maksymiec W. 2007. Signalling responses in plants to heavy metal stress. Acta Physiologiae Plantarum 29: 177-187.
• Nikolova M. Berkov S, and Ivancheva S. 2004. A rapid TLC method for analysis of external flavonoid aglycones in plant exudates. Acta Chromatographica 14: 110-114.
• Park JW, Shin YK, and Lee CS. 1991. The protective actions of rutin and quercetin on the Cu2+ plus H2O2 - induced collagen degradation. Korean Biochemistry Journal 24: 182-186.
• Ray SD, Guruprasad KN, and Laloraya MM. 1980. Antagonistic action of phenolic compounds on abscisic acid-induced inhibition of hypocotyl growth. Journal of Experimental Botany 31: 1651-1656.
• Saniewski M, Miyamoto K, and Ueda J. 1998. Methyl jasmonate influence gums and stimulates anthocyanin accumulation in peach shoots. Journal of Plant Growth Regulation 17: 121-124.
• Skórzyńska-Polit E, Drążkiewicz M, Wianowska D, Maksymiec W, Dawidowicz AL, and Tukiendorf A. 2004. The influence of heavy metal stress on the level of some flavonols in the primary leaves of Phaseolus coccineus. Acta Physiologiae Plantarum 26: 247-254.
• Stoynova-Bakalova E, and Petrov P. 2006. Control by cytokinins of the cellular behaviour in the plate meristem of zucchini cotyledons. Planta 223: 1256-1263.
• Stoynova-Bakalova E. 2007. Properties of plate meristem of growing epigeal cotyledons in an experimental system. Environmental and Experimental Botany 59: 76-83.
• Stoynova-Bakalova E, Petrov PI, Gigova L, and Baskin TI. 2008. Effects of methyl jasmonate on cell growth and division of etiolated Cucurbita pepo (zucchini) cotyledon. Plant Biology 10: 276-284.
• Tamari G, Borochov A, Atzorn R, and Weiss D. 1995. Methyl jasmonate induces pigmentation and flavonoid gene expression in Petunia corollas: A possible role in wound response. Physiology Plantarum 94: 45-50.
• Wasternack C. 2007. Jasmonates: An update on biosynthesis, signal transduction and action in plant stress response, growth and development. Annals of Botany (London) 100: 681-697.
• Xiang C, and Oliver DJ.1998. Glutathione metabolic genes coordinately respond to heavy metals and jasmonic acid in Arabidopsis. Plant Cell 10: 1539-1550.
• Yang Y, Zheng H, Li Y, Ji J-X, and Yu L-J. 2008. Effects of methyl jasmonate and methyl dihydrojasmonate on the cell growth and flavonoids accumulation in cell suspension culture of Glycyrrhiza inflata bat. Plant Physiology Communications 44: 903-906.
• Yoshitama K. 2000. Recent advances in secondary metabolism research: regulation of biosynthesis and physiological functions of flavonoids and some phenolics. Journal of Plant Research 113: 285.