The role of reactive nitrogen species and cigarette smoke in activation of transcription factor NF-kappaB and implication to inflammatory processes

• Autorzy: Bar-Shai M. , Hasnis E. , Wiener-Megnazi Z. , Reznick A.Z.
• Języki publikacji: EN

Abstrakty

EN

Using the electromobility shift assay (EMSA) in the rat myoblast system, the activation of transcription factor NF-B by reactive nitrogen species was evaluated. Two distinct patterns of activation were demonstrated. Whereas NO donor, SNAP, activated NF-B in the classical pathway, which led to a transient response, NF-B activation by peroxynitrite donor, SIN-1, was mediated by an alternative pathway, which has been demonstrated in previous works to involve tyrosine nitration of the NF-B inhibitory protein I-Balpha. This led to a constitutive non-transient activation of NF-B and a prolonged inflammatory reaction. Lymphocytes exposed to mild intensity of cigarette smoke for 8 h, which activated NF-B, exhibited a decrease in the fraction of apoptotic cells from 27% to 19% compared with lymphocytes exposed to atmospheric air, using the FACS Annexin V assay. This also has been shown in previous works to be mediated by peroxynitrite. Thus, mild exposure to cigarette smoke induces NF-B activation, which can attenuate apoptosis in human lymphocytes and lead to prolonged inflammatory response. A possible proposed mechanism for induction of chronic inflammatory response may involve peroxynitrite-induced activation of NF-B.

Słowa kluczowe

EN

peroxynitrite; myoblast; cigarette smoke; reactive oxygen species; nitric oxide; nuclear factor-kappaB; lymphocyte; apoptosis; rat; reactive nitrogen species

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2006
• Tom: 57
• Numer: 4
• Strony: 39-44
• Opis fizyczny: p.39-44,fig.,ref.

Twórcy

autor

Bar-Shai M.

• Technion-Israel Institution of Technology, Haifa, 31096 Israel

autor

Hasnis E.

autor

Wiener-Megnazi Z.

autor

Reznick A.Z.