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The paper showed the applicability of the PCR method to an early identification of Fusarium avenaceum and Fusarium culmorum in infected tissues of selected crops. The polymerisation chain reaction used species-specific SCAR primers. There was observed a discrepancy between the size of multiplied DNA of Fusarium avenaceum fragment and that of Fa-U17f 5, Fa-U17r earlier described by authors of primers. The present research product size for F. avenaceum was 950 bp, while for F. culmorum - 472 bp which confirmed the reports by other authors.
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http://www.ejpau.media.pl/series/volume4/issue2/agronomy/art-02.html
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- Warmia and Masuria University, 5 Plac Lodzki, 10-957 Olsztyn, Poland
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Bibliografia
- Chełkowski J., Witkowska I., 1999. Identification of fungal pathogens in cereals and genetic pathogen diversity with the PCR. Post. Nauk. Rol. 4, 49-60 [in Polish].
- Narayanasamy P., 1997. Plant pathogen detection and disease diagnosis. Marcel Dekker Inc., 182-189.
- Schaad N.W., Bonde M.R., Hatziloukas E., 1997. BIO-PCR: a highly sensitive technique for detecting seedborne fungi and bacteria. Seed Health Testing, 159-164.
- Shilling A.G., Möller E.M., Geiger H.H., 1996. Polymerase chain reaction-based assays for species-specific detection of Fusarium culmorum, F. graminearum and F. avenaceum. Phytopathology 86, 515522.
- Stevens E.A., Blakemore E.J.A., Reeves J.C., 1997. Development of a PCR-based test to detect and identify Pyrenophora spp. Seed Health Testing, 139-145.
- Turner A.S., Lees A.K., Rezanoor H.N., Nicholson P. 1998. Refinement of PCR-detection of Fusarium avenaceum and evidence from DNA marker studies for phenetic relatedness to Fusarium tricinctum. Plant Pathology 47, 278288.
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Bibliografia
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