Application of aqueous hydrazine solution for beta-elimination of O-glycans from gastric mucin

• Autorzy: Kisiel D. , Gindzienski A. , Gadek A.
• Języki publikacji: EN

Abstrakty

EN

O-Glycans from pig gastric mucin were released by β-elimination in 0.2 M triethylamine and 50% aqueous hydrazine water solution. The released glycan hydrazides were isolated using Centricon 10 separators, brought to their reducing form and reductive by labelled with p-aminobenzoic acid ethyl ester (ABEE). Labelled products were fractionated into neutral and acid fractions on a Bio-Gel P4 column, calibrated with a mixture of dextran oligosaccharides, labelled according to the same procedure.

Słowa kluczowe

EN

pig; O-glycan; beta-elimination; gastric mucin; p-aminobenzoic acid ethyl ester; dextran oligosaccharide; aqueous hydrazine

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 3
• Opis fizyczny: p.753-757,fig.

Twórcy

autor

Kisiel D.

• Medical Academy, A.Mickiewicza 2c, 15-230 Bialystok 8, Poland

autor

Gindzienski A.

autor

Gadek A.

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