Carbonic anhydrase activity in the vas deferens of the cotton leafworm - Spodoptera littoralis [Lepidoptera: Noctuidae] controlled by circadian clock

• Autorzy: Kotwica J. , Ciuk M.A. , Joachimiak E. , Rowinski S. , Cymborowski B. , Bebas P.
• Języki publikacji: EN

Abstrakty

EN

The male reproductive tract of Lepidoptera is an ideal model for the study of the physiological role of peripheral clocks in insects. The latter are significant in the generation and coordination of rhythmic phenomena which facilitate the initial stages of sperm capacitation. This process requires the maintenance of pH in the upper vas deferens (UVD) aided by, among others, H+-ATPase. Our aim was to determine the potential involvement of carbonic anhydrase (CA) in this process, an enzyme tasked with generating protons subsequently utilized by H+-ATPase to acidify the UVD milieu in S. littoralis, during the time when the lumen of this organ is filled with sperm. We attempted to answer the question whether CA activity can be controlled by the biological oscillator present in the male reproductive tract of the cotton leafworm. Using PAGE zymography, the presence of CA was demonstrated in the UVD wall, but not in the luminal fluid nor in the sperm. Using histochemistry, it was shown that CA is active in the UVD epithelium, and that this activity varies throughout the day and is most likely controlled by an endogenous biological clock. Conversely, the application of CA inhibitors, acetazolamide and sodium thiocyanate, in conjunction with an analysis of H+-ATPase activity in the acidification the UVD environment shows that CA most likely does not play a direct role in the regulation of the pH in this organ.

Słowa kluczowe

EN

carbonic anhydrase activity; cotton leafworm; Spodoptera littoralis; Lepidoptera; Noctuidae; circadian clock; sperm capacitation; acetazolamide; sodium thiocyanate; acidification; circadian rhythm; biological clock; sperm maturation; insect reproduction

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2006
• Tom: 57
• Numer: 8
• Strony: 107-123
• Opis fizyczny: p.107-123,fig.,ref.

Twórcy

autor

Kotwica J.

• Warsaw University, 1 Miecznikowa Str., 02-096 Warsaw, Poland

autor

Ciuk M.A.

autor

Joachimiak E.

autor

Rowinski S.

autor

Cymborowski B.

autor

Bebas P.