Analysis of the G-C polymorphism in the 5'-untranslated region of the RAD51 gene in breast cancer

• Autorzy: Blasiak J. , Przybylowska K. , Czechowska A. , Zadrozny M. , Pertynski T. , Rykala J. , Kolacinska A. , Morawiec Z. , Drzewoski J.
• Języki publikacji: EN

Abstrakty

EN

The breast cancer suppressor proteins BRCA1 and BRCA2 interact with RAD51, a protein essential for maintaining genomic stability by playing a central role in homology-dependent recombinational repair of the DNA double-strand breaks. Therefore, genetic variability in the RAD51 gene may contribute to the appearance and/or progression of breast cancer. A single nucleotide polymorphism in the 5 - un­translated region of RAD51 (a G to C substitution at position 135, the G/C polymor­phism) is reported to modulate breast cancer risk. We investigated the distribution of genotypes and frequency of alleles of the G/C polymorphism in breast cancer. Tumor tissues were obtained from postmenopausal women with node-negative and node-positive breast carcinoma with uniform tumor size. Blood samples from age matched healthy women served as control. The G/C polymorphism was determined by PCR-based MvaI restriction fragment length polymorphism. The distribution of the genotypes of the G/C polymorphism did not differ significantly (P > 0.05) from those predicted by the Hardy-Weinberg distribution. There were no differences in the geno­type distribution and allele frequencies between node-positive and node-negative pa­tients. There were no significant differences between distributions of the genotypes in subgroups assigned to histological grades according to Scarf–Bloom–Richardson criteria and the distribution predicted by Hardy–Weinberg equilibrium (P > 0.05). Our study implies that the G/C polymorphism of the RAD51 gene may not be directly involved in the development and/or progression of breast cancer and so it may not be useful as an independent marker in this disease.

Słowa kluczowe

EN

polymorphism; RAD51 gene; protein; breast; 5'-untranslated region; polymerase chain reaction; cancer; genetic polymorphism

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 1
• Opis fizyczny: p.249-253,fig.

Twórcy

autor

Blasiak J.

• University of Lodz, S.Banacha 12-16, 90-237 Lodz, Poland

autor

Przybylowska K.

autor

Czechowska A.

autor

Zadrozny M.

autor

Pertynski T.

autor

Rykala J.

autor

Kolacinska A.

autor

Morawiec Z.

autor

Drzewoski J.

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