Effect of sodium nitrate [V] on Saccharomyces cerevisiae strains of different antioxidative status and energetic metabolism

• Autorzy: Swiecilo A.

Warianty tytułu

PL

Oddzialywanie azotanu [V] sodu na szczepy drozdzy Saccharomyces cerevisiae o roznym statusie antyoksydacyjnym i metabolizmie energetycznym

• Języki publikacji: EN

Abstrakty

EN

The Saccharomyces cerevisiae yeast, differing with respect to the efficiency of antioxidating system and activity of mitochondrial processes, was used in the experiment. Sensitivity of these cells to 40-min incubation with sodium nitrate (V) was determined. Respiratory-competent cells deprived of the main antioxidating enzymes and the cells subjected to oxidative stress generated by antimycin A showed a greater sensitivity to sodium nitrate (V) than the cells deprived of functional mitochondria or the cells taken during the stationary phase of growth. The obtained results show that reactive oxygen species do not play an important part in the mechanisms of toxicity induced by the presence of sodium nitrates (V) in the case of cells with oxygen metabolism.

PL

Oddziaływanie azotanu (V) sodu badano na komórkach drożdży Saccharomyces cerevisiae różniących się sprawnością systemu antyoksydacyjnego i aktywnością procesów mitochondrialnych. Oznaczono wrażliwość tych komórek na 40 minutową inkubację z azotanem V sodu. Kompetentne oddechowo komórki pozbawione głównych enzymów antyokydacyjnych oraz komórki poddane stresowi oksydacyjnemu, generowanemu za pomocą antymycyny A charakteryzowały się większą wrażliwością na azotan V sodu niż komórki pozbawione funkcjonalnych mitochondriów lub pobrane ze stacjonarnej fazy wzrostu (rys. 1 i 2). Uzyskane wyniki sugerują, że reaktywne form tlenu nie odgrywają istotnej roli w mechanizmach toksyczności indukowanych obecnością azotanu V sodu w przypadku komórek o metabolizmie tlenowym.

Słowa kluczowe

EN

antioxidant activity; reactive oxygen species; yeast strain; dismutase activity; sodium nitrate; Saccharomyces cerevisiae; yeast cell; antioxidative system

• Wydawca: -
• Czasopismo: Polish Journal of Food and Nutrition Sciences
• Rocznik: 2008
• Tom: 58
• Numer: 1
• Opis fizyczny: p.41-44,fig.,ref.

Twórcy

autor

Swiecilo A.

• University of Agriculture of Lublin, Szczebrzeska 102, 22-400 Zamosc, Poland

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