Enterotoksynotworcze szczepy Bacteroides fragilis izolowane od koni

• Autorzy: Obuch-Woszczatynski P. , Pituch H. , Martirosian G. , Silva J. , Meisel-Mikolajczyk F. , Luczak M.
• Języki publikacji: PL

Abstrakty

PL

W próbkach różnych materiałów pobranych od koni poszukiwano entero- toksyno-twórczych szczepów Bacteroides fragilis (ETBF). Z badanych materiałów wyhodowano siedem szczepów B. fragilis. Do wykrywania fragmentu genu enterotoksyny (fragilizyny) zastosowano łańcuchową reakcję polimerazy (PCR) z użyciem starterów 404 i 407. Jako szczepów kontrolnych użyto szczepu enterotoksynotwórczego B. fragilis NCTC 11295 oraz nieenterotoksynotwórczego (NTBF) szczepu IPL 323. Obecność genu fragilizyny stwierdzono w dwóch szczepach. Udowodniono, że wśród szczepów izolowanych od koni można wykryć enterotoksynotwórcze szczepy B. fragilis.

EN

Seven Bacteroides fragilis strains were cultured from samples collected from horses. From alI the tested strains, as well as from the reference B. fragilis strains: enterotoxigenic NCTC 11925 and nonenterotoxigenic IPL 323 strain, DNA was isolated using Genomic DNA PREP PLUS isolation kit manufactured by A&A Biotechnology (Poland). To detect the enterotoxin (fragilysin) gene, polymerase chain reaction (PCR) was applied, using the following starters: 404 (GAG CCG AAG ACG GTG TAT GTG ATT TGT) and 407 (TGC TCA GCG CCC AGT ATA TGA CCT AGT). DNA obtained from bacterial cells was amplified in a thermocycler (Techne). The temperature profile was as follows: 1 cycle (4 min. 94°C), 40 cycles (1 min. 94°C, 1 min. 52°C, 1 min 74°C). Amplification products were detected by electrophoresis in agarose gel (1%) with ethidium bromide added. The presence of the fragilysin gene was detected in two strains. Among the strains isolated from horses enterotoxin gene-possesing Bacteroides fragilis strains (ETBF) can be detected.

Słowa kluczowe

PL

szczepy bakteryjne; Bacteroides fragilis; mikrobiologia lekarska; enterotoksyny; lancuchowa reakcja polimerazy; szczepy enterotoksynotworcze; bakterie; konie

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2001
• Tom: 53
• Numer: 2
• Strony: 161-166
• Opis fizyczny: s.161-166,fot.,tab.

Twórcy

autor

Obuch-Woszczatynski P.

• Akademia Medyczna, 02-004 Warszawa, ul.T.Chalubinskiego 5

autor

Pituch H.

autor

Martirosian G.

autor

Silva J.

autor

Meisel-Mikolajczyk F.

autor

Luczak M.

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