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The API 20E test and standard methods were applied to examine biochemical features of P. multocida strains. All tested isolates decomposed glucose, mannitol and saccharose. Sorbitol and arabinose were fermented by 82% and 43% of the strains, respectively. Xylose, trehalose, and dulcitol were fermented by 30%, 13% and 4% of isolates, respectively. All the tested strains produced catalase, ornithine decarboxylase, cytochrome-oxidase, indole, and reduced nitrates to nitrites. Moreover, the strains did not reveal motility and did not grow on MacConkey agar and failed to ferment maltose. Our findings enabled the recognition of ten distinct biochemical types, termed biovars I to X, and permitted the assignation of the strains into 3 subspecies. Of the isolates tested, 78% were identified as P. multocida subsp. multocida, 8.1 % as P. multocida subsp. septica, and 4% as P. multocida subsp. gallicida according to Mutters et al. ( 12). On the other hand, 9.7% of the strains were not classified as one of the three subspecies.
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p.161-167
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- National Veterinary Research Institute, 24-100 Pulawy, Poland
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Bibliografia
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- 2. Christensen J.P., Bisgaard M.: Avian pasteurellosis: taxonomy of the organisms involved and aspects of pathogenesis. Avian Path. 1997, 26, 461- 483.
- 3. Christensen J.P., Dietz H.H., Bisgaard M.: Phenotypic and genotypie characters of isolates of Pasteurella multocida obtained from back-yard poultry and from two outbreak of avian cholera in avifauna in Denmark. Avian Path. 1998, 27, 373 - 381.
- 4. Donahue J.M., Olson L.D.: Biochemic study of Pasteurella multocida from turkeys. Avian Dis. 1972, 16, 501 - 505.
- 5. Dorsey T.A.: Studies on fowl cholera. I. A biochemic study of avian Pasteurella multocida strains. Avian Dis. 1963, 7, 386 - 392.
- 6. Fegan N., Blackall P.J., Pahoff J.L.: Phenotypic characterisation of Pasteurella multocida isolates from Australian poultry. Vet. Microbiol. 1995, 47, 281 - 286.
- 7. Heddleston K.L.: Physiologic characteristics of 1268 cultures of Pasteurella multocida. Am. J. vet. Res. 1976, 37, 745 - 747.
- 8. Hirsh D.C., Jessup D.A., Snipes K.P., Carpenter T.E., Hird D.W., McCapes R.H.: Characteristics of Pasteurella multocida isolated from waterfowl and associated avian species in California. J. Wildl. Dis. 1990, 26, 204 - 209.
- 9. Kauc L.: Elektroforeza DNA w zmiennym (pulsowym) polu elektrycznym - PFGE. Post. Mikrobiol. 1992, 31, 35-56.
- 10. Murti P.S.R.C.: Studies on fowl cholera. I. Biochemical investigations of Pasteurella multocida. Acta vet. Acad. Sci. Hung 1971, 21, 313 - 317.
- 11. Mutters R., Ihm P., Pohl S., Mannheim W.: Reclassification of the genus Pasteurella Trevisan 1887 on the basis of deoxribonucleic acid homology, with proposals for new species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis, Pasteurella langaa. Int. J. syst. Bact. 1985, 35, 309 - 322.
- 12. Mutters R., Mannheim W., Bisgaard M.: Taxonomy of the group. In: Pasteurella and pasteurellosis, Ed. Adlam C., Rutter J.M., Acad. Press, London 1989, pp. 3 - 34.
- 13. Rutkowska-Jurga I. Borkowska-Opacka B.: Określanie przynależności serotypowej szczepów Pasteurella multocida wyizolowanych od drobiu. Medycyna wet., in print.
- 14. Snipes K.P., Hirh D.C., Kasten R. W., Carpenter T. E., Hird D. W., McCapes R.: Homogeneity of characteristics of Pasteurella multocida isolated from turkeys and wildlife in California. Avian Dis. 1990, 34, 315 -320.
- 15. Tarasiuk K., Truszczyński M.: Znaczenie fenotypowych i genotypowych metod klasyfikacji bakterii w epidemiologii i zwalczaniu chorób zakaźnych. Medycyna Wet, 1995, 51, 323-326.
- 16. Tenover F.C., Arbeit R.D., Goering R.V., Mickelsen P.A., Murray B.E., Persing D.H., Swaminathan B.: Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: Criteria for bacterial strain typing. J. Clin. Microbiol. 1995, 33, 2233-2239.
- 17. Tereszczukowa M.: Badanie nad właściwościami biologicznymi krajowych szczepów Pasteurella multocida, wyosobnionych z drobiu padłego na pasterelozę, pod kątem ich doboru do produkcji biopreparatów. Praca doktorska, Puławy 1974.
- 18. Walser M.M., Davis R.B.: In vitro characterization of field isolates of Pasteurella multocida from Georgia turkeys. Avian Dis. 1975, 19, 525 - 532.
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