Wplyw leukotoksyny Mannheimia haemolytica A1 na odpowiedz zapalna u cielat

• Autorzy: Bednarek D , Szymanska-Czerwinska M. , Dudek K.

Warianty tytułu

EN

Effect of leukotoxin Mannheimia haemolytica A1 on the inflammatory response in calves

• Języki publikacji: PL

Abstrakty

EN

The aim of this study was to estimate the effect of leukotoxin (Lkt) Mannheimia haemolytica A1 on the inflammatory response in calves. The study was performed on 12 clinically healthy, Black and White Lowland breed calves, aged 8 weeks. Each of the experimental animals received a single dose of 25µg of Mannheimnia haemolytica A1 Lkt intravenously. The Lkt treated calves were compared with non-treated controls before (0) and 1, 2, 3, 4, 5, 6, 12, 24, 48 and 72 h after the treatment. The following parameters were assayed: concentrations of eicosanoids such as: prostaglandins E₂ and F₂α (PGE₂, PGF₂α), thromboxaneB₂(TXB₂) and leukotriene B4 (LTB₄), as well as selected acute phase proteins (CRP, Cp, Tf, Hp, SAA). The obtained results showed that the concentrations of some acute phase proteins, such as Hp and SA,A and also eicosanoids (PGE₂, PGF2α, TXB₂, LTB₄), were significantly higher in comparison to the controls especially during the first 3-6 h after the Lkt administration.

Słowa kluczowe

PL

bydlo; choroby ukladu oddechowego; czynniki chorobotworcze; Mannheimia haemolytica A1; leukotoksyna; cieleta; odpowiedz zapalna

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2010
• Tom: 66
• Numer: 06
• Strony: 400-404
• Opis fizyczny: s.400-404,tab.,bibliogr.

Twórcy

autor

Bednarek D

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

autor

Szymanska-Czerwinska M.

autor

Dudek K.

Bibliografia

• 1.Bednarek D.: Badania nad modulacją odczynów zapalnych płuc w terapii cieląt chorych z objawami bronchopneumonii. Praca hab., PIWet-PIB, Puławy 2003.
• 2.Bednarek D.: Znaczenie zakażeń Pasteurella sp. w etiopatogenezie syndromu oddechowego bydła, [w:] Bednarek D. (red.): Najważniejsze czynniki etiologiczne, patogeneza i najnowsze trendy w profilaktyce i terapii syndromu oddechowego bydła (BRD). PIWet-PIB, Puławy 2008, 48-58.
• 3.Bednarek D., Urban-Chmiel R., Dudek K., Szymańska-Czerwińska M.: Evaluation of peripheral blood leukocyte subpopulations by flow cytometry in calves treated with Mannheimia haemolytica leukotoxin. Bull. Vet. Inst. Pulawy 2009, 53, 199-203.
• 4.Boosman R., Niewold T. A., Mutsaers C. W. A. A. M., Gruys E.: Serum amyloid A concentrations in cows given endotoxin as an phase stimulant. Am. J. Vet. Res. 1989, 50, 1690-1694.
• 5.Clinkenbeard K. D., Clinkenbeard C., Wawrzyniak B.: Choatropic agents cause disaggregation and enhabced activity of Pasteurella haemolytica leukotoxin. Vet. Microbiol. 1995, 45, 201-209.
• 6.Clinkenbeard K. D., Mosier D. A., Confer A. W.: Transmembrane pore size and role of cell swelling in cytotoxicity caused by Pasteurella haemolytica leukotoxin. Infect. Immun. 1989, 57, 420-425.
• 7.Conner J. G., Eckersall P. D., Wiseman A., Bain R. K., Douglas T. A.: Acute phase response in calves following infection with Pasteurella haemolytica, Ostertagia ostertagi and endotoxin administration. Res. Vet. Sci. 1989, 47, 203-207.
• 8.Deigan T., Alwan A., Kelly J., McNair J.,Warren T., O'Farelly C.: Serum haptoglobulin: an objective indicator of experimentally-induced Salmonella infection in calves. Res. Vet. Sci. 2000, 69, 153-158.
• 9.Godson D. L., Campos M., Attah-Poku S. K., Redmond M. J., Cordeiro D. M., Setih M. S., Harland R. J., Babiuk L. A.: Serum haptoglobin as an indicator of the acute phase response in bovine respiratory disease. Vet. Immunol. Immunopathol. 1996, 51, 277-292.
• 10.Heegard P. M. H., Godson D. L., Toussaint M. J. M., Tjornehoj K., Larsen L. E., Viuff B., Ronsholt L.: The acute phase of haptoglobulin and serum amyloid A (SAA) in cattle undergoing experimental infection with respiratory syncytial virus. Vet. Immunol. Immunopathol. 2000, 77, 151-159.
• 11.Hivronen J., Pyärölä S., Jousimies-Somer H.: Acute phase response in heifers with experimentally induced mastitis. J. Dairy Res. 1996, 64, 351-360.
• 12.Horadagoda A., Eckersall P. D., Alsemgeest S. P., Gibbs H. A.: Purification and quantitative measurement of bovine serum amyloid-A. Res. Vet. Sci. 1993, 55, 317-325.
• 13.Kaehler K. L., Markham R. J. F., Muscoplat Ch. C., Johson D. W.: Evidence of species specificity in the cytocidal effect of Pasteurella haemolytica. Infect. Immun. 1980, 30, 615-616.
• 14.Kostro K., Gliński Z.: Białka ostrej fazy u zwierząt. WAR, Lublin 2003.
• 15.Lawrence P. K., Nelson W. R., Liu W., Knowles D. P, Foreyt W. J., Sikumaran S.: β₂ integrin Mac-1 is a receptor for Mannheimia haemolytica leukotoxin on bovine and ovine leukocytes. Vet. Immunol. Immunopathol. 2008, 122, 285-294.
• 16.Mackiewicz S.: Immunologia. PZWL, Warszawa 1991.
• 17.Mikucki P., Wernicki A., Puchalski A., Urban-Chmiel R.: Skuteczność natywnej i inaktywowanej leukotoksyny Mannheimia haemolytica w immunoprofilaktyce swoistej syndromu oddechowego owiec. Medycyna Wet. 2006, 62, 1302-1305.
• 18.Nagabata H., Kebrli M. E., Murata H., Okada H., Noda H., Kociba G. J.: Neutrophil function and pathologic findings in Holstein calves with leukocyte adhesion deficiency. Am. J. Vet. Res. 1994, 55, 40-48.
• 19.Urban-Chmiel R., Wernicki A., Puchalski A., Mikucki P.: Evaluation of Mannheimia haemolytica leukotoxin preparated in non-supplemented and BSA or FBS supplemented RPMI 1640 medium. Pol. J. Vet. Sci. 2004, 7, 1-8.
• 20.Wittum T. E., Young C. R., Stanker L. H., Griffin D. D., Perino L. J., Littledike E. T.: Haptoglobulin response to clinical respiratory tract disease in feedlot cattle. Am. J. Vet. Res. 1996, 57, 646-649.