Short term 13-cis-retinoic acid treatment at therapeutic doses elevates expression of leptin, GLUT4, PPARgamma and aP2 in rat adipose tissue

• Autorzy: Krskova-Tybitanclova K. , Macejova D. , Brtko J. , Baculikova M. , Krizanova O. , Zorad S.
• Języki publikacji: EN

Abstrakty

EN

Temporary defects in the plasma lipid and glucose homeostasis are frequent complication accompanying chronic treatment with 13-cis-retinoic acid (13cRA). White adipose tissue acts as an endocrine organ producing a variety of hormones (adipocytokines) including leptin, adiponectin, tumor-necrosis factor alpha (TNF) and angiotensin II (Ang II), which influence lipid metabolism, systemic insulin sensitivity and inflammation. To study the effect of a short-term 13cRA administration on metabolism of epididymal fat tissue, we treated Wistar rats with five identical therapeutic doses of 13cRA (0.8 mg/kg b.w.) by gavage during a period of 10 days. Expression of adiponectin, leptin, TNF and selected proteins such as adipocyte fatty acid binding protein (aP2), insulin-dependent glucose transporter GLUT4, peroxisome proliferator-activated receptor gamma (PPAR) and retinoid X receptors (RXRs) was investigated using RT-PCR. Short-term treatment with therapeutic doses of 13cRA caused significant increase of the aP2, PPAR and moderately RXR gene expression. Similarly, the relative amount of mRNA for leptin and GLUT4 was increased, while the TNF transcript was decreased after treatment with 13cRA. The gene expression and plasma concentration of adiponectin were without any significant changes. Since local adipose renin-angiotensin system (RAS) has been presumed to be involved in the regulation of fat tissue metabolism, we also investigated the gene expression of RAS components in epididymal fat depot. Our data has shown that 13cRA elevated Ang II receptor type 1 (AT1 receptor) - at both, mRNA and protein level. Thus, our results demonstrate that short-term 13cRA treatment is inducing alterations in fat tissue metabolism in relation to stimulated adipogenesis.

Słowa kluczowe

EN

13-cis-retinoic acid; adipose tissue; nuclear receptor; renin-angiotensin system; tumour necrosis factor-alpha; angiotensin; rat; hormone; adipocytokine; adiponectin; endocrine organ; leptin; retinoic acid

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2008
• Tom: 59
• Numer: 4
• Strony: 731-743
• Opis fizyczny: p.731-743,fig.,ref.

Twórcy

autor

Krskova-Tybitanclova K.

• Slovak Academy of Sciences, Vlarska 3, 833 06 Bratislava, Slovakia

autor

Macejova D.

autor

Brtko J.

autor

Baculikova M.

autor

Krizanova O.

autor

Zorad S.