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2006 | 62 | 02 | 231-233
Tytuł artykułu

Zmiany w ekspresji izoform bialka tau w mozgu myszy w zaleznosci od wieku

Warianty tytułu
Języki publikacji
PL
Abstrakty
EN
au is a microtubule-associated protein important for the assembly and stabilization of microtubules. Six tau isoforms are produced in the central nervous system from one single gene as a result of the alternative splicing of exons 2, 3 (N-terminal part) and exon 10 (C-terminal part). The shortest isoform (2-3-10-, 0N 3R) is characteristic for fetal brains, whereas the remaining (2+3-10-, 1N 3R; 2+3+10-, 2N 3R; 2-3-10+, 0N 4R; 2+3-10+, 1N 4R; 2+3+10+, 2N 4R) for adult brains. The aim of the study was to establish a profile of tau protein variants in the C57BL/6J mouse frontal cortex during the aging process. The total RNA was isolated from tissues, followed by reverse transcription and PCR reaction. It was found that the sequence encoded by exon 10 was absent in the youngest 5-day old newborns (isoform 3R), while it was present in 21, 70 and 140-day old animals (isoform 4R). The most abundant isoform in 5-day old mice was 1N and accounted for 66% of the total tau protein. The percentage of 1N isoforms lowered with age and was 31% in 140-day old animals. The total percentage of 0N isoforms was 11% in 5-day old mice and was approximately threefold lower than in each of the older groups. It may be concluded that alternative splicing of the tau protein undergoes age-dependent regulation in the mouse brain cortex.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
62
Numer
02
Strony
231-233
Opis fizyczny
s.231-233,rys.,bibliogr.
Twórcy
autor
  • Akademia Medyczna, ul. Banacha 1, 02-097 Warszawa
autor
autor
autor
Bibliografia
  • 1.Andreadis A., Brown W. M., Kosik K. S.: Structure and novel exons of the human tau gene. Biochemistry 1992, 31, 10626-10633.
  • 2.Brandt R., Lee G.: Functional organization of microtubule-associated protein tau. J. Biol. Chem. 1993, 268, 3414-3419.
  • 3.Brandt R., Leger J., Lee G.: Interaction of tau with the neural plasma membrane mediated by tau's amino-terminal projection domain. J. Cell Biol. 1995, 131, 1327-1340.
  • 4.Drechsel D. N., Hyman A. A., Cobb M. H., Kirschner M. W.: Modulation of the dynamic instability of tubulin assembly by the microtubule-associated protein tau. Mol. Biol. Cell 1992, 3, 1141-1154.
  • 5.Goedert M.: Tau protein and neurodegeneration. Seminars in Cell&Developmental Biology 2004, 15, 45-49.
  • 6.Georgieff I. S., Liem R. K., Couchie D., Mavilia C., Nunez J., Shelanski M. L.: Expression of high molecular weight tau in the central and peripheral nervous systems. J. Cell Sci. 1993, 105, 729-737.
  • 7.Hwang S. C., Jhon D. Y., Bae Y. S., Kim J. H., Rhee S. G.: Activation of phospholipase C-gamma by the concerted action of tau proteins and arachidonic acid. J. Biol. Chem. 1996, 271, 18342-18349.
  • 8.Kosik K. S., Orecchio L. D., Bakalis S., Neve R. L.: Developmentally regulated expression of specific tau sequences. Neuron 1989, 2, 1389-1397.
  • 9.Lee G., Neve R. L., Kosik K. S.: The microtubule binding domain of tau protein. Neuron 1989, 2, 1615-1624.
  • 10.Lee G., Newman S. T., Gard D. L., Band H., Panchamoorthy G.: Tau interacts with src-family non-receptor tyrosine kinases. J. Cell Sci. 1998, 111, 3167-3177.
  • 11.Panda D., Goode B. L., Feinstein S. C., Wilson L.: Kinetic stabilization of microtubule dynamics at steady state by tau and microtubule-binding domains of tau. Biochemistry 1995, 34, 11117-11127.
  • 12.Pizzi M., Valerio A., Belloni M., Arrighi V., Alberici A., Liberini P., Spano F., Memo M.: Differential expression of fetal and mature tau isoforms in primary cultures of rat cerebellar granule cells during differentiation in vitro. Molecular Brain Research 1995, 34, 38-44.
  • 13.Schoenfeld T. A., Obar R. A.: Diverse distribution and function of fibrous microtubule-associated proteins in the nervous system. Int. Rev. Cytol. 1994, 151, 67-137.
  • 14.Spillantini M. G., Goedert M.: Tau protein pathology in neurodegenerative diseases. Trends Neurosci. 1998, 21, 428-433.
  • 15.Tawana K., Ramsden D. B.: Progressive supranuclear palsy. Mol. Pathol. 2001, 54, 427-434.
  • 16.Weingarten M. D., Lockwood A. H., Hwo S. Y., Kirschner M. W.: A protein factor essential for microtubule assembly. Proc. Natl. Acad. Sci. USA 1975, 72, 1858-1862.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-article-0c689645-1d85-4180-a370-0d9e44aeaea3
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