The influence of bolaamphiphilic steroid dimer on the formation and structure of bilayer lipid membranes

• Autorzy: Kalinowski S. , Lotowski Z. , Morzycki J.W.
• Języki publikacji: EN

Abstrakty

EN

The process of self-assembly and the electromechanical properties of bilayer lipid membranes (BLM) were investigated. The membranes were made of phosphatidylcholine with the addition of bolaamphiphilic steroid dimer. The membranes were formed using the Mueller-Rudin method. Membrane formation in the presence of the dimer was much faster and they were more stable than those formed in the absence of the dimer. The membranes formed by this method usually contain residues of a solvent used in the formation process which increases membrane thickness. Thus, the membranes formed from pure phosphatidylcholine had an average thickness of 5.9 nm. The addition of steroid dimer to the forming solution caused the thickness to decrease to 3.9 nm. An external voltage applied to the bilayer lipid membranes caused electrocompression. The presence of bolaamphiphilic steroid dimer in the membranes decreased the electrocompressibility by approximately 20 times. The dimer molecules „spanned” both monolayers of the membranes and caused the membrane thickness to decrease during their formation. The presence of the dimer in the membrane limited the mobility of solvent inside the membrane. The membranes formed with the dimer have such properties as thickness, stability, resistance, breakdown voltage, electrocompressibility, and time of formation more adequate for their application as a biomembrane model and support for sensors based on biomembrane molecules.

Słowa kluczowe

EN

Mueller-Rudin method; electromechanical property; lipid membrane; membrane formation; bilayer lipid membrane; membrane formation process; bolaamphiphilic steroid dimer; electrocompression; phosphatidylcholine

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2000
• Tom: 05
• Numer: 1
• Opis fizyczny: p.107-118,fig.

Twórcy

autor

Kalinowski S.

• Warmia and Masuria University in Olsztyn, 10-957 Olsztyn, Poland

autor

Lotowski Z.

autor

Morzycki J.W.

Bibliografia

• 1. Gennis, R. B. Biomembranes: Molecular Structure and Functions, (Cantor, C. R., Ed.), Springer-Verlag, New York, 1989.
• 2. Hanke, W. and Schlue, W. R. Planar Lipid Bilayers: Methods and Applications, (Sattelle, D. B., Ed), Academic Press, London, 1993.
• 3. Blum, L. J. and Coulet, P. R. Biosensors: New tools in analytical chemistry. Electroanalysis, 20 (1992) M34-M37.
• 4. Tien, H. T., Barish, R. H., Gu, L. Q. and Ottova, A. L. Supported bilayer lipid membranes as ion and molecular probes. Anal. Sci. 14 (1998) 3-14.
• 5. Tapuchi, E. 1991. Molecular electronics: A new interdisciplinary field of research. Interdiscipl. Sci. Rev. 16 (1991) 45-60.
• 6. Mueller, P., Rudin, D. O., Tien H. T. and Wescott, W. C. Methods for the formation of single bimolecular lipid membranes in aqueous solutions. J. Phys. Chem. 67 (1963) 534-535.
• 7. Fettiplace, R., Gordon, L. G. M., Hladky, S. B., Requena, J., Zingsheim, H. P. and Haydon, D. A. Techniques in the formation and examination of ’’black” lipid bilayer membranes. Methods in Membrane Biology, vol. 4 (Korn, E. D., Ed.), Plenum Press, New York, 1975, 1-75.
• 8. White, S. H. and Thompson, T. E. Capacitance, area, and thickness variations in thin lipid films. Biochim. Biophys. Acta 323 (1973) 7-22.
• 9. Stern, J., Freisleben, H. J., Janku, S. and Ring, K. Black lipid membranes of tetraether lipids from Thermoplasma acidophilum. Biochim. Biophys. Acta 1128 (1992) 227-236.
• 10. Gambacorta, A., Gliozzi, A., De Rosa, M. Archaeal lipids and their biotechnological applications. World J. Microbiol. Biotechnol. 11 (1995) 115-131.
• 11. Relini, A., Cassinadri, D., Fan, Q., Gulik, A., Mirghani, Z., De Rosa, M. and Gliozzi, A. Effect of physical constrains on the mechanisms of membrane fusion: bolaform lipid vesicles as model systems. Biophys. J., 71 (1996) 1789-1795.
• 12. Rhodes, D. G. and A. Singh. Structure of polymerizable lipid bilayers IV: mixtures of long chain diacetylenic and short chain saturated phosphatidylcholines and analogous asymmetric isomers. Chem. Phys. Lipids 59 (1991) 215-224.
• 13. Janas, T., Kotowski, J. and Tien, H. T. 1988. Polymer-modified bilayer lipid membranes: the polypyrrole-lecithin system. Bioelectrochem. Bioenerg. 19 (1988) 405-412.
• 14. Yeagle P. L., 1985. Cholesterol and the cell membrane. Biochim. Biophys. Acta 822 (1985) 267-287.
• 15. Tien, H. T. Bilayer Lipid Membranes: Theory and Practice. Marcel Dekker, Inc., New York, 1974.
• 16. Morzycki, J. W., Kalinowski, S., Łotowski, Z. and Rabiczko, J. Synthesis of dimeric steroids as components of lipid membranes. Tetrahedron 53 (1997) 10579-10590.
• 17. Kalinowski,S. And Figaszewski Z. A four-electrode system for measurement of bilayer lipid membrane capacitance. Meas. Sci. Technol. 6 (1995) 1043-1049.
• 18. Kalinowski,S. and Figaszewski Z. A four-electrode potentiostat- galvanostat. Meas. Sci. Technol. 6 (1995) 1050-1055.
• 19. Kalinowski, S. and Figaszewski, Z. A new system for bilayer lipid membrane capacitance measurements: method, apparatus and applications. Biochim. Biophys. Acta 1112 (1992) 57-66.
• 20. Dilger, J. P. and Benz, R. Optical and electrical properties of thin monoolein lipid bilayers. J. Membrane Biol. 85 (1985) 181-189.
• 21. White S. H. Formation of ’’solvent-free” black lipid bilayer membranes from glyceryl monooleate dispersed in squalene. Biophys. J. 23 (1978) 337-347.
• 22. Schoch, P., Sargent, D. F. and Schwyzer, R. Capacitance and conductance as tool for the measurement of asymmetric surface potentials and energy barriers of lipid bilayer membranes. J. Membrane Biol. 46 (1979) 71-89. ,
• 23. Sargent, D. F. and Hianik, T. Comparative analysis of the methods for; measurement of membrane surface potential of planar lipid bilayer. Bioelectrochem. Bioenerg. 33 (1994) 11-18.
• 24. Kalinowski, S., Ibron, G., Bryl, K. and Figaszewski, Z. Chronopotentiometric studies of electroporation of bilayer lipid membranes. Biochim. Biophys. Acta 1369 (1998) 204-212.
• 25. Kalinowski, S. Influence of polarisation potential on capacitance of bilayer lipid membranes. PhD Thesis, Warsaw University, Warsaw, 1997.