Application of specific primers in the diagnosis of Encephalitozoon spp.

• Autorzy: Valencakova A. , Balent P. , Novotny F. , Cislakova L.
• Języki publikacji: EN

Abstrakty

EN

In our experiment, 3 species-specific primer pairs cultivated in cell lines were used: Encephalitozoon cuniculi-specific primer pairs (ECUNF and ECUNR), Encephalitozoon hellem-specific primer pairs (EHELF and EHELR), and Encephalitozoon intestinalis-specific primer pairs (SINTF and SINTR). The PCR products were estimated to be 550 bp in E. cuniculi, 547 bp in E. hellem and 545 bp in E. intestinalis respectively, which can prove the precision and reliability of this method in the species identification of the genus Encephalitozoon. All 3 primer pairs were species-specific and none of them amplified gene sequences from other Encephalitozoon spp.

Słowa kluczowe

EN

encephalitozoonosis; microsporidian infection; parasite; Encephalitozoon cuniculi; Encephalitozoon intestinalis; Encephalitozoon hellem; polymerase chain reaction; diagnosis; specific primer

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2005
• Tom: 12
• Numer: 2
• Strony: 321-323
• Opis fizyczny: p.321-323,ref.

Twórcy

autor

Valencakova A.

• University of Veterinary Medicine, Komenskeho 73, 041 81, Kosice, Slovak Republic

autor

Balent P.

autor

Novotny F.

autor

Cislakova L.

Bibliografia

• 1. Chu P, West AB: Encephalitozoon (Septata) intestinalis. Cytologic, histologic, and electron microscopic features of a systemic intestinal pathogen. Am J Clin Pathol 1996,106, 606-614.
• 2. Da Silva AJ, Slemenda SB, Visvesvara GS, Schwartz DA, Wilcox CM, Wallace S, Pieniążek NJ: Detection of Septata intestinalis (Microsporidia) Cali et al. 1993 Using Polymerase Chain Reaction Primers Targeting the Small Submit Subunit Ribosomal RNA Coding Region. Mol Diagn 1997, 2, 47-52.
• 3. Fedorko DP, Hijazi YM: Application of molecular techniques to the diagnosis of microsporidial infections. Emerging Infect Dis 1996, 2, 183-192.
• 4. Franzen C, Schwartz DA, Visvesvara GS, Müller A, Schwenk A, Salzberger B, Fätkenheuer G, Hartmann P, Mahrle G, Diehl V, Schrappe M: Immunologically confirmed disseminated, asymptomatic Encephalitozoon cuniculi infection of the gastrointestinal tract in a patient with AIDS. Clin Infect Dis 1995, 21, 1480-1484.
• 5. Hartskeerl RA, Schuitema AR, De Wächter R: Secondary structure of the small subunit ribosomal RNA sequence of the microsporidium Encephalitozoon cuniculi. Nucleic Acids Res 1993, 21, 1489.
• 6. Scaglia M, Sacchi L, Gatti S, Bernuzzi AM, Polver P, Piacentini I, Concia E, Croppo GP, Da Silva AJ, Pieniążek NJ, Slemenda SB, Wallace S, Leitch GJ, Visvesvara GS: Isolation and identification of Encephalitozoon hellem from an Italian AIDS patient with disseminated microsporidiosis. APMIS 1994,102, 817-827.
• 7. Scaglia M, Sacchi L, Croppo GP, Da Silva A, Gatti S, Corona S, Orani A, Bernuzzi MA, Pieniążek NJ, Slemenda SB, Wallace S, Visvesvara GS: Pulmonary microsporidiosis due to Encephalitozoon hellem in a patient with AIDS. J Infect 1997, 34, 119-126.
• 8. Valenčáková A, Bálent P, Malčeková B, Lesník F, Halánová M, Hipíková V: Comparison of mammalian microsporidia species replication in various cell lines. Biologia 2002, 57(6), 773-776.
• 9. Visvesvara GS, Leitch GJ, Da Silva AJ, Croppo GP, Moura H, Wallace S, Slemenda SB, Schwartz DA, Moss D, Bryan RT, Pieni¹¿ek NJ: Polyclonal and monoclonal antibody and PCR-amplified small- subunit rRNA identification of a microsporidian, Encephalitozoon hellem, isolated from an AIDS patient with disseminated infection. J Clin Microbiol 1994, 32, 2760-2768.
• 10. Vossbrinck CR, Maddox JV, Friedman S, Debrunner-Vossbrinck В A, Woese CR: Ribosomal RNA sequence suggests microsporidia are extremely ancient eukaryotes. Nature 1987, 326, 411-414.
• 11. Vossbrinck CR, Baker MD, Didier ES, Debrunner-Vossbrinck BA, Shadduck JA: Ribosomal DNA sequences of Encephalitozoon hellem and Encephalitozoon cuniculi: species identification and phylogenetic construction. J Eukaryot Microbiol 1993, 40, 354-362.
• 12. Zhu X, Wittner M, Tanowitz HB, Cali A, Weiss LM: Nucleotide sequence of the small ribosomal RNA of Encephalitozoon cuniculi. Nucleic Acids Res 1993,21, 1315.