Molecular and morphological evidence for natural hybridization between Primula secundiflora Franchet and P. poissonii Franchet [Primulaceae]

• Autorzy: Zhu X -F , Li Y. , Wu G.-L. , Fang Z.-D. , Li Q.-J. , Liu J.-Q.
• Języki publikacji: EN

Abstrakty

EN

Natural hybridization occurs commonly in plants and blurs their interspecific delimitation. It is unclear whether spontaneous hybridization occurs in natural populations of Primula in China. In this study we examined natural hybridization between P. secundiflora and P. poissonii based on morphological and molecular analyses of individuals with intermediate morphology. Most morphological characters of these individuals were found to be intermediate between the putative parental species, but plant height and corolla tube length were significantly transgressive. Molecular analyses based on nuclear internal transcribed spacer (ITS) additivity and two types of cloned sequences (each corresponding respectively to one of two parental species) clearly suggested that these individuals result from hybridization between P. secundiflora and P. poissonii. We further used a maternally inherited chloroplast DNA fragment (rpl16 intron) to trace the maternal composition of the hybrids. Among 26 hybrids, ten (two thrums, eight pins) had the P. secundiflora haplotype, while 16 (ten thrums, six pins) possessed the P. poissonii haplotype. These results suggest that both parents served as the mother donors of the hybrids. Reciprocal hybridization between the two species seems to be symmetrical rather than unidirectional

Słowa kluczowe

EN

molecular evidence; morphological evidence; natural hybridization; hybridization; Primula secundiflora; Primula poissonii; Primulaceae

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 2009
• Tom: 51
• Numer: 2
• Strony: 29-36
• Opis fizyczny: p.29-36,fig.,ref.

Twórcy

autor

Zhu X -F

• Lanzhou University, Lanzhou 73000, China

autor

Li Y.

autor

Wu G.-L.

autor

Fang Z.-D.

autor

Li Q.-J.

autor

Liu J.-Q.

Bibliografia

• Anderson E. 1949. Introgressive Hybridization. Wiley, New York
• Arnold ML. 1992. Natural hybridization as an evolutionary process. Annual Review of Ecology and Systematics 23: 237-261.
• Arnold ML. 1997. Natural Hybridization and Evolution. Oxford University Press, New York.
• Barrett SCH, and Cruzan MB. 1994. Incompatibility in heterostylous plants. In: Williams EG, Knox RB, Clark AE [eds.], Genetic Control of Incompatibility and Reproductive Development in Flowering Plants, 189-219. Kluwer Academic Publishers, Netherlands.
• Baum DA, Small RL, and Wendel JF. 1998. Biogeography and floral evolution of baobabs (Adansonia, Bombacaceae) as inferred from multiple data sets. Systematic Biology 47: 181-207.
• Bleeker W, and Hurka H. 2001. Introgressive hybridization in Rorippa (Brassicaceae): gene flow and its consequences in natural and anthropogenic habitats. Molecular Ecology 10: 2013-2022.
• Broyles SB. 2002. Hybrid bridges to gene flow: a case study in milkweeds (Asclepias). Evolution 56: 1943-1953.
• Clifford HT. 1958. Studies in British primulas. VI. On introgression between primrose (Primula vulgaris Huds.) and cowslip (P. veris L.). New Phytologist 57: 1-10.
• Doyle J. 1991. DNA protocols for plants - CTAB total DNA isolation. In: Hewitt GM and Johnston A [eds.], Molecular Techniques in Taxonomy, 283-293.Springer, Berlin.
• Ellstrand NC, Whitkus R, and Rieseberg LH. 1996. Distribution of spontaneous plant hybrids. Proceeding of the National Academy of Science USA 93: 5090-5093.
• Guggisberg A, Baroux C, Grossniklaus U, and Conti E. 2008. Genomic origin and organization of the allopolyploid Primula egaliksensis investigated by in situ hybridization. Annals of Botany 101: 919-927.
• Guggisberg A, Mansion G, Kelso S, and Conti E. 2006. Evolution of biogeographic patterns, ploidy levels, and breeding systems in a diploid-polyploid species complex of Primula. New Phytologist 171: 617-632.
• Harris SA, and Ingram R. 1991. Chloroplast DNA and biosys- tematics: the effects of intraspecific diversity and plastid transmission. Taxon 40: 393-412.
• Hu CM. 1990. Primulaceae. In: Flora Reipublicae Popularis Sinicae, vol. 27. Science Press, Beijing.
• Hu CM. 1994. On the geographical distribution of the Primulaceae. Journal of Tropical and Subtropical Botany 2: 1-14.
• Jordan WC, Courtney MW, and Neigel JE. 1996. Low levels of intraspecific genetic variation at a rapidly evolving chloroplast DNA locus in North American duckweeds (Lemnaceae). American Journal of Botany 83: 430-439.
• Kálmán K, Medvegy A, Pénzes Z, and Mihalik E. 2007. Morphspecific variation of flora traits association with reciprocal herkogamy in natural population of Primula vulgaris and Primula veris. Plant Systematic and Evolution 268: 15-27.
• Kelso S. 1991. Taxonomy of Primula sects. Aleuritia and Armerina in North America. Rhodora 93: 67-99.
• Kelso S. 1992. The genus Primula as a model for evolution in the Alaskan flora. Arctic Antarctic and Alpine Research 24: 82-87.
• Lamont BB, He T, Enright NJ, Krauss SL, and Miller BP. 2003. Anthropogenic disturbance promotes hybridization between Banksia species by altering their biology. Journal of Evolution Biology 16: 551-557.
• Li XJ, Wang LY, Yang HL, and Liu JQ. 2007. Confirmation of natural hybrids between Gentiana straminea and G. siphonantha (Gentianaceae) based on molecular evidence. Acta Botanica Yunnanica 29: 91-97.
• Mast AR, Kelso S, and Conti E. 2006. Are any primroses (Primula) primitively monomorphic? New Phytologist 171: 605-616.
• Mast AR, Kelso S, Richards AJ, Lang DJ, Feller DMS, and Conti E. 2001. Phylogenetic relationships in Primula L. and related genera (Primulaceae) based on noncoding chloroplast DNA. International Journal of Plant Science 162: 1381-1400.
• Mogensen HL. 1996. The hows and whys of cytoplasmic inheritance in seed plants. American Journal of Botany 83: 383-404.
• Mullis KB, and Faloona FA. 1987. Specific synthesis of DNA in vitro via a polymerase catalyzed chain reaction. Methods in Enzymology 155: 335-350.
• Noyes RD. 2006. Intraspecific nuclear ribosomal DNA divergence and reticulation in sexual diploid Erigeron strigosus (Asteraceae). American Journal of Botany 93: 470-479.
• Olmstead RG, and Palmer JD. 1994. Chloroplast DNA sys- tematics: a review of methods and data analysis. American Journal of Botany 81: 1205-1224.
• Qiu S, Zhou RC, Li YQ, Havanond S, Jaengjai C, and Shi SH. 2008. Molecular evidence for natural hybridization between Sonneratia alba and S. grffithii. Journal of Systematics and Evolution 46: 391-395.
• Richards J. 2002. Primula. New edition. BT Batsford Ltd, London.
• Richards JH, and Koptur S. 1993. Floral variation and distyly in Guettarda scabra (Rubiaceae). American Journal of Botany 80: 31-40.
• Rieseberg LH, and Carney SE. 1998. Plant hybridization. New Phytologist 140: 599-624.
• Rieseberg LH, and Wendel JF. 1993. Introgression and its consequences in plants. In: Harrison RG [ed. ], Hybrid Zones and the Evolutionary Process, 70-109. Oxford University Press, Oxford.
• Sang T, Crawford DJ, and Stuessy TF. 1995. Documentation of reticulate evolution in peonies (Paeonia) using internal transcribed spacer sequences of nuclear ribosomal DNA: implications for biogeography and evolution. Proceedings of the National Academy of Sciences USA 92: 6813-6817.
• SPSS Inc. 2001. Statistic Analysis Software of Large database: SPSS 11.0 for Windows. USA.
• Stace CA. 1975. Hybridization and the Flora of the British Isles. Academic Press, London, UK.
• Stebbins GL. 1950. Variation and Evolution in Plants. Columbia University Press, New York.
• Stebbins GL. 1957. Self-fertilization and population variability in the higher plants. The American Naturalist 91: 337-354.
• Stebbins GL. 1959. The role of hybridization in evolution. Proceeding of American Philosophical Society 103: 231-251.
• Thompson JD, and Lumaret R. 1992. The evolutionary dynamics of polyploidy plants: origins, establishment and persistence. Trends in Ecology and Evolution 7: 302-307.
• Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, and Higgins DG. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25: 4876-4882.
• Tiffin P, Olson MS, and Moyle LC, 2001. Asymmetrical crossing barriers in angiosperms. Proceeding of the Royal Society B-Biological Sciences 268: 861-867.
• Tovrsanchez E, and Oyama K. 2004. Natural hybridization and hybrid zones between Quercus crassifolia and Quercus crassipes (Fagaceae) in Mexico: morphological and molecular evidence. American Journal of Botany 91: 1352-1363.
• Turelli M, and Moyle LC. 2007. Asymmetric postmating isolation: Darwin's corollary to Haldane's rule. Genetics 176: 1059-1088.
• Valentine DH. 1961. Evolution in the genus Primula. In: Wanstall PJ [ed.], A Darwin Centenary, 71-87. Botanical Society of the British Isles, London, UK.
• van der Velde M, and Bijlsma R. 2004. Hybridization and asymmetric reproductive isolation between the closely related bryophyte taxa Polytrichum commune and P. uliginosum. Molecular Ecology 13: 1447-1454.
• Wang FY, Gong X, Hu CM, and Hao G. 2008. Phylogeography of an alpine species Primula secundiflora inferred from the chloroplast DNA sequence variation. Journal of Systematic and Evolution 46: 13-22.
• Webb CJ, and Lloyd DG. 1986. The avoidance of interference between the presentation of pollen and stigmas in angiosperms II. Herkogamy. New Zealand Journal of Botany 24: 163-178.
• Wedderburn F, and Richards AJ. 1990. Variation in withinmorph incompatibility inhibition sites in heteromorphic Primula L. New Phytologist 116: 149-162.
• White TJ, Bruns T, Lee S, and Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M, Gelfand D, and Sninský J [eds.], PCR protocols: A Guide to Methods and Application, 315-322. Academic Press, San Diego.
• Woodell SRJ. 1960. Studies in British Primulas VIII. Development of seed from reciprocal crosses between P. vulgaris Huds. and P. elatior (L.) Hill; and between P. veris L. and P. elatior (L.) Hill. New Phytologist 59: 314-325.
• Woodell SRJ. 1965. Natural hybridization between the cowslip (Primula veris L.) and the primrose (P. vulgaris Huds.) in Britain. Watsonia 6: 190-202.
• Woodell SRJ. 1969. Natural hybridization in Britain between Primula vulgaris Huds. (the Primrose) and P. elatior (the Oxlip). Watsonia 7: 115-127.
• Wu CA, and Campbell DR. 2005. Cytoplasmic and nuclear markers reveal contrasting patterns of spatial genetic structure in a natural Ipomopsis hybrid zone. Molecular Ecology 14: 781-792.
• Xiang L, Werth CR, Emery SN, and McCauley DE. 2000. Population-specific gender-biased hybridization between Dryopteris intermedia and D. carthusiana: evidence from chloroplast DNA. American Journal of Botany 87: 1175-1180.
• Zhang HG, Richard IM, and Sun H. 2008. Morphological and molecular evidence of natural hybridization between two distantly related Rhododendron species from the Sino- Himalaya. Botanical Journal of the Linnean Society 156: 119-129.
• Zhou Re, Gong X, Boufford D, Wu CI, and Shi SH. 2008. Testing a hypothesis of unidirectional hybridization in plants: observations on Sonneratia, Bruguiera and Ligularia. BMC Evolutionary Biology 8: 149.
• Zhu HF, Yang JB, Zhang CQ and Li DZ. 2002. Systematic position of Primula secundiflora (Primulaceae) inferred from nuclear ribosomal DNA ITS sequence data. Acta Phytotaxonomica Sinica 40: 133-138.
• Zhu HF, Zhang CQ, Gu ZJ, and Gong X. 2001. A karyomorphological study on nine species of Primula (Primulaceae). Acta Botanica Yunnanica 23: 466-472.