Evidence for essential histidine and dicarboxylic amino-acid residues in the active site of UDP-glucose: solasodine glucosyltransferase from eggplant leaves

• Autorzy: Nawloka P. , Kalinowska M. , Paczkowski C. , Wojciechowski Z.A.
• Języki publikacji: EN

Abstrakty

EN

Effects of several chemical probes selectively modifying various amino-acid resi­dues on the activity of UDP-glucose : solasodine glucosyltransferase from eggplant leaves was studied. It was shown that diethylpyrocarbonate (DEPC), a specific modi­fier of histidine residues, was strongly inhibitory. However, in the presence of exces­sive amounts of the enzyme substrates, i.e. either UDP-glucose or solasodine, the in­hibitory effect of DEPC was much weaker indicating that histidine (or histidines) are present in the active site of the enzyme. Our results suggest also that unmodified resi­dues of glutamic (or aspartic) acid, lysine, cysteine, tyrosine and tryptophan are nec­essary for full activity of the enzyme. Reagents modifying serine and arginine resi­dues have no effect on the enzyme activity.

Słowa kluczowe

EN

garden eggplant; solasodine; glucosyltransferase; glycoalkaloid; Solanaceae; Solanum melongena; enzyme activity; leaf; amino acid; active site

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 2
• Opis fizyczny: p.567-572,fig.

Twórcy

autor

Nawloka P.

• Warsaw University, I.Miecznikowa 1, 02-096 Warsaw, Poland

autor

Kalinowska M.

autor

Paczkowski C.

autor

Wojciechowski Z.A.

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