Oxidative stress induces IGF-1 receptor signaling disturbances in cultured human dermal fibroblasts. A possible mechanism for collagen biosynthesis inhibition

• Autorzy: Sienkiewicz P , Palka M. , Palka J.
• Języki publikacji: EN

Abstrakty

EN

The effects of oxidative stress on collagen and DNA biosynthesis, b- galactosidase and prolidase activities, and the expression of prolidase, b1- integrin receptor, FAK, IGF-IR and MAP-kinases (ERK1, ERK2) were evaluated in human dermal fibroblasts. Subconfluent cells were subjected to repetitive stresses with 30mM t-BHP for 1 hour per day over the course of 5 days. It was found that oxidative stress induced the inhibition of collagen biosynthesis in these cells in a time-dependent manner. Exposure of the cells to 5 stresses contributed to a decrease in collagen and DNA biosynthesis to about 30% and 50% of the control values, respectively. Prolidase activity and expression were only suppressed in fibroblasts subjected to 1 and 3 stresses. In these cells prolidase activity was decreased by about 20%. As a result of 5 stresses, no further inhibition of prolidase activity occurred; however, expression of the enzyme was slightly increased, as demonstrated by Western blot analysis. It was found that these phenomena were neither related to the expression of b1-integrin receptor nor to that of FAK. However, the exposure of the cells to 3 and 5 stresses contributed to a distinct decrease in IGF-IR and MAP-kinases (ERK1, ERK2) expression, which is probably responsible for the collagen biosynthesis inhibition.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2004
• Tom: 09
• Numer: 4A
• Strony: 643-650
• Opis fizyczny: p.643-650,fig.,ref.

Twórcy

autor

Sienkiewicz P

• Medical Academy of Bialystok, Kilinskiego 1, 15-230 Bialystok, Poland

autor

Palka M.

autor

Palka J.

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