Czasopismo
Tytuł artykułu
Autorzy
Warianty tytułu
Embryo transfer in sheep as a method for controlling MVV infection
Języki publikacji
Abstrakty
The transmission of maedi-visna wirus (MVV) through embryons collected from virus infected ewes and then transferred to uninfected recipients was investigated. Early sheep embryos were isolated from four in vivo-fertilized, MVV infected donors, which were determined to be positive by ELISA and PCR. A total of fifty-two morula and blastocyst stage embryos were washed ten times and transferred (two or three per recipient) to twenty-two serologically negative sheep. Three ewes became pregnant, delivering three live kids out of which one died immediately after delivery. All two kids were then kept over 72-weeks-of-age and neither MVV specific antibodies nor proviral DNA was found in the blood samples collected before colostrum suckling, as well as after 14, 32, 56 and 72-weeks-of-age. Additionally, no active virus particules were found when tested by syncytia formation assay. This study demonstrated that it is possible to obtain transferable stage embryos from donor ewes infected with MVV, in vivo fertilized by infected rams, and that collected embryos are unlikely to transmit MVV infection to the resulting offspring.
Wydawca
Czasopismo
Rocznik
Tom
Numer
Strony
942-945
Opis fizyczny
s.942-945,rys.,tab.,bibliogr.
Twórcy
autor
- Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy
autor
autor
autor
autor
Bibliografia
- 1.Bielanski A., Nadin-Davis S., Simard C., Maxwell P., Algire J.: Experimental collection and transfer of embryos from bovine immunodeficiency virus (BIV) infected cattle. Theriogenology 2001, 55, 641-648.
- 2.Bielanski A., Simard C., Maxwell P., Nadin-Davis S.: Bovine immunodeficiency virus in relation to embryos fertilized in vitro. Vet. Res. Commun. 2001, 25, 663-673.
- 3.Bielanski A., Maxwell P., Simard C.: Effect of bovine leukaemia virus on embryonic development and association with in vitro fertilised embryos. Vet. Rec. 2000, 146, 255-256.
- 4.Brodie S., Pearson L., Snowder G., DeMartini.: Host-virus interaction as defined by amplification of viral DNA and serology in lentivirus-infected sheep. Arch.Virol. 1992, 130, 413-428.
- 5.Fieni F., Rowe J., Burucoa C., Oppenheim S., Anderson G., Merray J., BonDurant R.: Presence of caprine arthritis-encephalitis virus (CAEV) infected cells in flushing media following oviductal-stage embryo collection. Theriogenology 2002, 57, 931-940.
- 6.Kumak J., Rola M. Kozaczyñska B.: Ocena metod serologicznych w diagnostyce zaka¿eñ wirusem maedi-visna u owiec. Medycyna Wet. 2002, 58, 444-447.
- 7.Lamara A., Fieni F., Mselli-Lakhal L., Chatagnon G., Bruyas J. F., Tainturier D., Battut I., Fornazero C., Chebloune Y.: Early embryonic cells from in vivo-produced goat embryos transmit the caprine erthritis-encephalitis virus (CAEV). Theriogenology 2002, 8629, 1-11.
- 8.Lamara A., Fieni F., Mselli-Lakhal L., Tainturier D., Chebloune Y.: Epithelial cells from goat oviduct are highly permissive for productive infection with caprine erthritis-encephalitis virus (CAEV). Virus Res. 2002.
- 9.Lamara A., Fieni F., Chebloune Y., Taintuurier D.: Efficient replication of caprine arthritis-encephalitis virus in goat granulosa cells. Virus Res. 2001, 79, 165-172.
- 10.Stringfellow D., Riddell K., Zurovac O.: The potential of embryo transfer for infectious disease control in livestock. N. Zel. Vet. J. 1991, 39, 8-17.
- 11.Thibier M., Guerin B.: Embryo transfer in small ruminants: the method of choice for health control in germplasm exchanges. Liv. Prod. Science 2000, 62, 253-270.
- 12.Wolfe D. F., Nusbaum K. E., Lauerman L. H., Mysinger P. W., Riddell M. G., Putman M. R., Shumway L. S., Powe T. A.: Embryo transfer from goats seropositive for caprine arthritis-encephalitis virus. Theriogenology 1987, 28, 307- -316.
- 13.Woodal C. J., Mylne J., McKelvie W. A. C., Watt N. J.: A technique for the sequential isolation of RNA and DNA from embryos developed for screening for viruses. J. Virol. Methods 1994, 46, 263-274.
- 14.Wrathall A. E.: Research on pathogens of importance in sheep and goats. Int. Embryo Movement Symp. Montreal, August, 1988, IETS, pp. 123-125.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-02bc51a6-40e4-48f9-8def-551e49ea3d36