Signals mediating Klotho-induced neuroprotection in hippocampal neuronal cells

• Autorzy: Cheng M.-F. , Chen L.-J. , Niu H.-S. , Yang T.-T. , Lin K.-C. , Cheng J.-T.
• Języki publikacji: EN

Abstrakty

EN

The erythropoietin (Epo) receptor (EpoR) is expressed in the brain and was shown to have neuroprotective effects against brain damage in animal models. A recent study indicated that EpoR and its activity are the downstream effectors of Klotho for cytoprotection in the kidney. Thus, we propose that Klotho can stimulate the expression of EpoR in neuronal cells to enhance Epo-mediated protection. H19-7 hippocampal neuronal cells were treated with recombinant Klotho. In H19-7 cells, Klotho increased the expression of both the EpoR protein and mRNA. Klotho also enhanced the transcription activity of the EpoR promoter in H19-7 cells. Moreover, Klotho augmented the Epo-triggered phosphorylation of Jak2 and Stat5 and protected H19-7 cells from hydrogen peroxide cytotoxicity. The silencing of EpoR abolished the protective effect of Klotho against peroxide-induced cytotoxicity. Finally, the silencing of GATA1 diminished the Klotho-induced increase in EpoR protein and mRNA expression as well as its promoter activity. In conclusion, Klotho increased EpoR expression in neuronal cells through GATA1, thereby enabling EpoR to function as a cytoprotective protein against oxidative injury.

Słowa kluczowe

EN

signal; Klotho protein; Klotho gene; neuroprotection; hippocampus; neuronal cell; brain damage; animal model

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: 1
• Opis fizyczny: p.60-71,fig.,ref.

Twórcy

autor

Cheng M.-F.

• Division of Nephrology, Department of Internal Medicine College of Medicine, National Cheng Kung University, Tainan City, Taiwan

autor

Chen L.-J.

• Institute of Basic Medical Science, National Cheng Kung University, Tainan City, Taiwan
• Department of Nursing, Tzu Chi College of Technology, Hualien City, Taiwan

autor

Niu H.-S.

• Department of Nursing, Tzu Chi College of Technology, Hualien City, Taiwan

autor

Yang T.-T.

• The School of Chinese Medicine for Post-Baccalaureate, I-Shou University, Kaohsiung City, Taiwan

autor

Lin K.-C.

• Department of Neurology, Chi-Mei Medical Center, Yong Kang, Tainan City, Taiwan
• Institute of Biotechnology, Southern Taiwan University, Yong Kang, Tainan City, Taiwan

autor

Cheng J.-T.

• Department of Medical Research, Chi-Mei Medical Center, Yong Kang, Tainan City, Taiwan
• Institute of Medical Sciences, Chang Jung Christian University, Gueiren, Tainan City, Taiwan

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