Callus induction and Organogenesis in Sugarcane (Saccharum officinarum L.) var 93v297

• Autorzy: Rao A.S.
• Języki publikacji: EN

Abstrakty

EN

An efficient protocol for induction of callus and regeneration of a sugar cane var 93v297 has been developed and reported here. Callus induction from immature young leaf explants derived from 2-3-month-old plants was achieved on Murashige and Skoog’s (MS) medium supplemented with different auxins viz, 2,4-D, NAA and IAA. Among different auxins, 2, 4-D at 3.5mg/l + 0.5mg/l BAP was found favourable in inducing callus. Addition of coconut milk and BAP further enhanced the growth of callus maximum being on MS medium supplemented with 0.5mg/l BAP (3602.33±0.88mg). Calli were further evaluated for regeneration. MS medium supplemented with 1.0 mg/l BAP was found suitable where 100% calli regenerated with maximum number of multiple shoots per callus mass (41.40±0.89). Highest number of root emergence (28.33±1.16) and maximum root length (3.40±0.67cm) was achieved on MS medium supplemented with 3mgl/l NAA. The in vitro grown plants were transferred to polycups containing a mixture of sterilized sand, soil and cocopeet (1:1:1) for hardening. The hardened plants were transferred to green-house conditions where they survived with 90% frequency.

Słowa kluczowe

EN

sugar-cane; Saccharum officinarum; callus; callus induction; organogenesis; rhizogenesis; leaf explant; growth regulator; auxin; cytokinin

• Wydawca: -
• Czasopismo: International Letters of Natural Sciences
• Rocznik: 2015
• Tom: 48
• Opis fizyczny: p.14-22,fig.,ref.

Twórcy

autor

Rao A.S.

• Plant tissue Culture and Genetic Engineering Laboratory, Department of P. G. Studies and Research in Botany, Gulbarga University, Kalaburagi- 585 106

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Identyfikatory

DOI

10.18052/www.scipress.com/ILNS.48.14