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2014 | 58 | 3 |
Tytuł artykułu

Haemotropic mycoplasma infection revealed by real - time PCR in specific pathogen - free rats

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The presence of Mycoplasma haemomuris (haemoplasma) in blood samples collected from specific pathogen-free (SPF) laboratory rats bred in Japan was reported. Its presence was examined in Fischer 344, Sprague-Dawley (SD), and Wistar rat strains of both sexes by real-time PCR. All strains were positive for M. haemomuris infection. The 16S rRNA gene of M haemomuris strain detected in the animals was amplified using end-point PCR. Only the entire nucleotide sequence of 16S rRNA gene of a mycoplasma strain detected in SD rats was determined and compared to those of other haemoplasmas. Our investigations suggest a wide M. haemomuris infection among the SPF rats purchased from commercial breeders in Japan.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
58
Numer
3
Opis fizyczny
p.375-378,fig.,ref.
Twórcy
autor
  • Cooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan
autor
  • Cooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan
autor
  • Cooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan
autor
  • Cooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan
autor
  • Cooperative Department of Veterinary Medicine, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan
Bibliografia
  • 1. Amici D., Murri O., Paparelli M., Tedeschi G.G.: Transmissione della Haemobartonella muris della madre al feto. Boll Soc Ital Biol Sper 1966, 42, 1501-1503.
  • 2. Baker H.J., Cassell G.H., Lindsev J.R.: Research complications due to Haemobartonella and Eperythrozoon infections in experimental animals. Am J Pathol 1971, 64, 625-652.
  • 3. Bartlett R., Pease P.: Latent Haemobartonella muris infection: its transmission and decline in an inbred, ectoparasite-free strain of Wistar rat. Parasitology 1975, 71, 35-40.
  • 4. Crystal M.M.: The mechanism of transmission of Haemobartonella muris (Mayer) of rats by the spined rat louse, Polyplax spinulosa (Burmeister). J Parasitol 1958, 44, 603-606.
  • 5. Kimura M.: A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980, 16, 111-120.
  • 6. Kreier J.P., Hall L.: The relationship of parasitemia to the life span of erythrocytes of rats infected with Haemobartonella muris. J Infect Dis 1968, 118, 443-448.
  • 7. Messick J.B.: Hemotrophic mycoplasmas (hemoplasmas): a review and new insights into pathogenic potential. Vet Clin Pathol 2004, 33, 2-13.
  • 8. Moore D.H., Arison R.N., Tanaka H., Hall W.T., Chanowitz M.: Identity of the filterable hemolytic anemia agent of Sacks with Haemobartonella muris. J Bacteriol 1965, 90, 1669-1674.
  • 9. Nishizawa I., Sato M., Fujihara M., Sato S., Harasawa R.: Differential detection of hemotropic Mycoplasma species in cattle by melting curve analysis of PCR products. J Vet Med Sci 2010, 72, 1704.
  • 10. Rikihisa Y., Kawahara M., Wen B., Kociba G., Fuerst P., Kawamori F., Suto C., Shibata S., Futohashi M.: Western immunoblot analysis of Haemobartonella muris and comparison of 16S rRNA gene sequences of H. muris, H. felis, and Eperythrozoon suis. J Clin Microbiol 1997, 35, 823-829.
  • 11. Saitou N., Nei M.: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987, 4, 406-425.
  • 12. Thompson J.D., Higgins D.G., Gibson T.J.: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994, 22, 4673-4680.
  • 13. Woods R., Parbhoo S.P.: Haemolytic anaemia in the Gunn rat following transplantation of isolated hepatocytes injected with Haemobartonella muris. Br J Exp Pathol 1981, 62, 8-12.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-9e86df7c-5da7-4b24-b1d9-56dbe73996fb
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