Quantitative determination of secoiridoid and xanthone glycosides of Gentiana dinarica Beck cultured in vitro

• Autorzy: Branka V , Dijana K.-M. , Teodora J. , Snezana Z.-K. , Dragan V.
• Języki publikacji: EN

Abstrakty

EN

Gentiana dinarica Beck, native to the Balkan Dinaric Mountains, was established in vitro from axillary shoot buds. It was maintained in the form of shoot cultures on MS medium supplemented with 1.0 mg l-1 6-benzyladenine (BA) and 0.1 mg l-1 a-naphthaleneacetic acid and excised root cultures were maintained on MS medium with 0.5 mg l-1 indole-3-butyric acid (IBA). Shoot cultures, adventitious roots and excised root cultures were analysed by HPLC techniques for the presence of secoiridoids and xanthones. Gentiopicrin and swertiamarin, the dominant components of shoot cultures, could not be detected in root cultures. Xanthones were present in both shoot and root cultures with norswertianin-1-O-primeveroside as the dominant metabolite. The secoiridoid and xanthone content, although characteristic for certain plant organs, was dependent on the concentration of plant growth regulators (BA and IBA) added to the medium. BA in the shoot multiplication stage strongly increased the secondary metabolite (SEM) content of shoot cultures. IBA had little effect on SEM accumulation in shoots during rooting, while it moderately stimulated SEM accumulation in excised root cultures.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 02
• Opis fizyczny: p.567-574,fig.,ref.

Twórcy

autor

Branka V

• Institute for Biological Research ‘‘Sinisˇa Stankovic´’’, University of Belgrade, Bul. Despota Stefana 142, 11000 Belgrade, Serbia

autor

Dijana K.-M.

• Institute for Biological Research ‘‘Sinisˇa Stankovic´’’, University of Belgrade, Bul. Despota Stefana 142, 11000 Belgrade, Serbia

autor

Teodora J.

• Institute for Medicinal Plant Research ‘‘Dr Josif Pancˇic´’’, Tadeusˇa Kosˇc´usˇka 1, 11000 Belgrade, Serbia

autor

Snezana Z.-K.

• Institute for Biological Research ‘‘Sinisˇa Stankovic´’’, University of Belgrade, Bul. Despota Stefana 142, 11000 Belgrade, Serbia

autor

Dragan V.

• Institute for Biological Research ‘‘Sinisˇa Stankovic´’’, University of Belgrade, Bul. Despota Stefana 142, 11000 Belgrade, Serbia

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