Expression of SP, CGRP, NOS, VIP and GAL in the porcine NG neurons supplying prepyloric stomach region following acetylsalicylic acid supplementation and partial stomach resection

• Autorzy: Calka J. , Rytel L.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: We analyzed expression of SP, CGRP, NOS, VIP and GAL in the porcine nodose ganglion (NG) sensory neurons innervating prepyloric stomach region in physiological state, following gastritis evoked by acetylsalicylic acid supplementation and after partial stomach resection. METHODS: The study was performed on 12 immature gilts divided into 3 groups (n=4 each). All animals were injected retrograde marker Fast Blue (FB) into the anterior prepyloric stomach wall. Since 7th day after FB injection animals of the ASA group were given acetylsalicylic acid (100 mg/kg BW) orally for 21 days, while animals of RES group underwent partial stomach resection on 22nd day following FB injection. On 28th day all pigs were euthanized and bilateral right (rNG) and left (lNG) were collected. Cryostat sections were double immunolabeled using antibodies against SP, CGRP, NOS, VIP and GAL. RESULTS: Microscopic analysis of the control group revealed expression of SP (45.9±3.4% in rNG and 60.4±1.7% in lNG), CGRP (32.5±4.3% in rNG and 42.6±9.5% in lNG), nNOS (34.9±6.8% in rNG and 49.9±9.3% in lNG), VIP (17.9±2.7% in rNG and 31.5±5.1% in lNG) and GAL (21.9±3.3% in rNG and 35.5±6.8% in lNG) in FB+ perykaria. In RES group increased expression of SP (61.3±3.3% in rNG and 55.2±5.6% in lNG), CGRP (60.6±2.7% in rNG and 52.8±3.7% in lNG), nNOS (49.3±4.5% in rNG and 52.8±9.0% in lNG), VIP (61.3±2.5% in rNG and 67.1±6.8% in lNG) and GAL (35.1±7.3% in rNG and 37.1±5.8% in lNG) in FB+ perykaria was found. In ASA group increased expression of SP (56.5±3.4% in rNG and 67.2±3.9% in lNG), CGRP (52.8±6.9% in rNG and 64.8±6.2% in lNG), nNOS (47.9±9.0% in rNG and 59.3±7.1% in lNG), VIP (51.9±6.0% in rNG and 60.3±5.6% in lNG), GAL (47.2±7.2% in rNG and 55.8±4.2% in lNG) in FB+ perykaria was observed. CONCLUSIONS: Our data suggest that SP, CGRP, NOS, VIP and GAL play a role in sensory transduction, posttraumatic dendrites regeneration, and development of the stomach.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S66

Twórcy

autor

Calka J.

• Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland

autor

Rytel L.

• Department of Clinical Physiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland