The influence of IL-10 on the inflammatory reaction changes in the mice after 1-methyl-4-phenyl-1,2,3,6-tertahydropyridine (MPTP) treatment

• Autorzy: Sznejder A. , Joniec-Maciejak I. , Ciesielska A. , Schwenkgrub J. , Wawer A. , Bankiewicz K. , Czlonkowska A. , Czlonkowski A.
• Języki publikacji: EN

Abstrakty

EN

Parkinson’s disease (PD) is one of most frequent neurological disorder characterized by the loss of dopaminergic neurons in substantia nigra and striatum. The typical reaction of central neural system (CNS) on neurodegenerative processes is microglia activation and the inflammatory reaction. Microglia activation stimulates astrocytes response, playing important role in neuroimmune reaction. Microglia cells secrete two types of mediators of the inflammatory process: anti- and pro- inflammatory. In the first stage of Parkinson’s disease, pro-inflammatory cytokines have important meaning. We investigated the effect of an adenoassociated viral vector (AAV2) containing the complementary DNA (cDNA) for human interleukine 10 (hIL-10). The aim of the present study was to examine the evaluation of inflammatory reaction changes following increased concentration of hIL-10 in the murine model of PD induced by MPTP. Male C57BL mice 12 month-old were used in this study. AAV2 vector was bilateraly administered into striatum at 7, 21, 28 days prior to MPTP intoxication. We observed changes in the morphology of microglia cells, infiltration of lymphocytes T (population of CD3+, CD4+ and CD8+) and some differences in the level of one of the most important pro inflammatory cytokines – IL-1α. Our study showed that IL-10 is strongly involved in the inflammatory reaction in the murine model of Parkinson’s disease induced by MPTP. After MPTP intoxication we observed the increase of activated microglia cells, infiltration of lymphocytes T and higher level of IL-1α mRNA. AAV2-hIL-10-treated mice displayed a significant decrease in the activated microglia cells, elevated expression of IL-10 receptors observed on glia cells, strong infiltration of lymphocytes T (mainly CD4+ and CD3+, less CD8+) and minor expression of IL-1α mRNA. Further research must be conducted to provide more evidence of protective role of IL-10 in Parkinson disease.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.71-72

Twórcy

autor

Sznejder A.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland

autor

Joniec-Maciejak I.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland

autor

Ciesielska A.

• Department of Neurosurgery, University of California San Francisco, San Francisco, USA

autor

Schwenkgrub J.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland

autor

Wawer A.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland

autor

Bankiewicz K.

• Department of Neurosurgery, University of California San Francisco, San Francisco, USA

autor

Czlonkowska A.

• Second Department of Neurology, Institute of Psychiatry and Neurology, Warsaw, Poland

autor

Czlonkowski A.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland

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