Cytotoxic effects of the synthetic oestrogens and androgens on Balb/c 3T3 and HepG2 cells

• Autorzy: Minta M. , Radko L. , Stypula-Trebas S. , Zmudzki J.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was to test and compare the cytotoxic potential of two synthetic oestrogens: diethylstilboestrol (DES) and ethinyloestradiol (EE₂) and two androgens: testosterone propionate (TP) and trenbolone (TREN) on two cell lines. The fibroblast cell line Balb/c 3T3 and the hepatoma cell line HepG2 were selected. To get more insight into the mode of toxic action, four methods were used, which evaluated different biochemical endpoints: mitochondrial activity (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide reduction assay), lysosomal activity (neutral red uptake assay), total protein content, and lactate dehydrogenase release. Cytotoxicity was assessed after 24, 48, and 72 h exposure to eight concentrations ranging from 0.78 to 100 µg/mL. Concentration- and time- dependent effects were observed. Depending on the line and assay used, half maximal effective concentration after 72 h (EC₅₀₋₇₂ₕ) values ranged as follows: DES 1-13.7 µg/mL (Balb/c 3T3) and 3.7-5.2 µg/mL (HepG2); EE₂ 2.1-14.3 µg/mL (Balb/c 3T3) and 1.8-7.8 µg/mL (HepG2); TP-14.9-17.5 µg/mL (Balb/c 3T3), and 63.9- 100 µg/mL (HepG2); and TREN 11.3-31.4 µg/mL (Balb/c 3T3) and 12.5-59.4 µg/mL (HepG2). The results revealed that oestrogens were more toxic than androgens and the most affected endpoint was mitochondrial activity. In contrast to oestrogens, for which EC₅₀₋₇₂ₕ values were similar in both lines and by all assays used, Balb/c 3T3 cells were more sensitive than HepG2 cells to TP.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2014
• Tom: 58
• Numer: 4
• Opis fizyczny: p.613-620,fig.,ref.

Twórcy

autor

Minta M.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Radko L.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Stypula-Trebas S.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Zmudzki J.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

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