Warianty tytułu
Języki publikacji
Abstrakty
The aim of the study was to determine the aetiologic agent causing deaths in two flocks of Pekin ducklings at the age of 12 d in Poland, on the basis of clinical symptoms and pathological changes, viral hepatitis infection was suspected in the birds. During the necropsy, liver sections were collected, from which total cellular RNA was isolated. Then, real-time polymerase chain reaction (RT-PCR) was performed using primers complementan to the pre-S region of the duck hepatitis virus genome. In all liver samples, the presence of a 530 bp PCR product was detected. The RT-PCR demonstrated the presence of genetic material of duck hepatitis virus type 1 (DH type 1 ) in the examined ducklings.
Słowa kluczowe
Wydawca
Rocznik
Tom
Numer
Opis fizyczny
p.513-515,fig.,ref.
Twórcy
autor
- Department of Poultry Viral Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
- Department of Poultry Viral Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
- Veterinary Clinic SPEC–DROB, 83-400 Koscierzyna, Poland
Bibliografia
- 1. Anchun C., Mingshu W., Hongyi X., Dekang Z., Xinran L., Haijuen C., Renyong J., Miao Y.: Development and application of a reverse transcriptase polymerase chain reaction to detect Chinese isolates of duck hepatitis virus type 1. J Microbiol Methods 2009, 77, 332-336.
- 2. Calnek B.W.: Duck virus hepatitis. In: Virus Infections of birds, edited by McFerran and M.S. McNulty, Elsevier Science Publishers B.V., Amsterdam, 1993, pp. 485-278.
- 3. Chen L., Ma M., Zhang R., Xu Q., Si X., Xie Z., Jiang S.: Simultaneous detection of duck hepatitis A virus ty pes 1 and 3, and of duck astrovirus type 1, by multiplex RT-PCR. Virol Sin 2014, 29, 196-198.
- 4. Ding C., Zhang D.: Molecular analysis of duck hepatitis virus type 1. Virology 2007, 361, 9-17.
- 5. Duck viral hepatitis. OIE Terrestrial Manual 2010, Chapter 2.3.8.
- 6. Jin X., Zhang W., Zhang W., Gu Ch., Cheng G., Hu X.: Identification and molecular analysis of the highly pathogenic duck hepatitis virus type 1 in Hubei province in China. Res Vet Sci 2008, 85, 595-598.
- 7. Kim M.C., Kwon Y.K., Joh S.J., Lindberg A.M., Kwon J.H., Kim J.H., Kim S.J.: Molecular analysis of duck hepatitis virus type 1 reveals a novel lineage close to the genus Parechovirus in the family Picornaviridae. J Gen Virol 2006, 87, 3307-3316.
- 8. Liu M., Fanyi-Meng, Li X.J., Zhang Z., Liu S., Zhang Y.: Goose haemorrhagic hepatitis caused by a new subtype duck hepatitis type 1 virus. Vet Microbiol 2011, 28, 290-293.
- 9. Marek K.: Duck viral hepatitis in Poland. Bulletin of the 2nd Congress of PTN W, Wroclaw, 1962, p. 207.
- 10. Wang L., Pan M., Fu Y., Zhang D.: Classification of ducks hepatitis virus into three genotypes based on molecular evolutionary analysis. Virus Genes 2008, 37, 52-59.
- 11. Zhang H., Pi J., Tang C., Yue H., Yang F.: An experimental study of the pathogenicity of a duck hepatitis A virus genotype C isolate in specific pathogen free ducklings. Avian Pathol 2012, 41, 613-620.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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