Cadmium’s effect on the organization of microtubular cytoskeleton in root tips cells of salix matsudana koidz

• Autorzy: Zou J. , Wang G. , Ji J. , Wang J. , Ouyang J. , Li B.
• Języki publikacji: EN

Abstrakty

EN

The toxic effects of Cd on microtubule (MT) organization in root tip cells of S. matsudana were investigated in the present study using tubulin immunolabeling and fluorescence microscopy. Cell damage and expression level of the SmTUA1 gene in the root tips were also examined by means of propidium iodide (PI) staining and quantitative real-time PCR (qRT-PCR) technology. The MT arrays were very sensitive to Cd. At interphase, under 50 μmol/L Cd treatment for 48 h, some cortical MTs were discontinuous, inducing numbers of differently sized fragments. With increased Cd concentrations and duration of treatment, peripheral MTs appeared to be broken gradually, and the degree of disorder was enhanced. Spindle fibers even formed condensed MT at 10 μmol/L Cd for 48 h. During anaphase/telophase, there was a small part of MT absent, MT fibers were stuck to each other (even forming lumps) and could not form phragmoplast at 50 μmol/L Cd for 48 h. The cell damage of S. matsudana root tips increased with enhanced Cd concentrations and prolonged treatment time. Expression level of SmTUA1 analyzed by qRT-PCR further validated the results by indirect immunofluorescence staining. The data obtained here will be very useful to understand the mechanisms of Cd-induced cell toxicity.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2018
• Tom: 27
• Numer: 2
• Opis fizyczny: P.939-945,fig.,ref.

Twórcy

autor

Zou J.

• School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, P.R. China
• Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, P.R. China

autor

Wang G.

• School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, P.R. China

autor

Ji J.

• School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, P.R. China

autor

Wang J.

• Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, P.R. China

autor

Ouyang J.

• Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, P.R. China

autor

Li B.

• Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin 300387, P.R. China

Bibliografia

• 1. CAO F.B., CHEN F., SUN H.Y., ZHANG G.P., CHEN Z.H., WU F.B. Genome-wide transcriptome and functional analysis of two contrasting genotypes reveals key genes for cadmium tolerance in barley. BMC Genomics 15, 1, 2014.
• 2. WU H.F., WANG J.Y., LI B.B., OU Y.J., WANG J.R., SHI Q.Y., JIANG W.S., LIU D.H., ZOU J.H. Salix matsudana Koidz tolerance mechanisms to Cadmium: uptake and accumulation, subcellular distribution, and chemical forms. Pol. J. Environ. Stud. 25, 1739, 2016.
• 3. JIANG Z., QIN R., ZHANG H.H., ZOU J.H., SHI Q.Y., WANG J.R., JIANG W.S., LIU D.H. Determination of Pb genotoxic effects in Allium cepa root cells by fluorescent probe, microtubular immunofluorescence and comet assay. Plant Soil 383, 357, 2014.
• 4. SHI Q.Y., WANG J.R., ZOU J.H., JIANG Z., WU H.F., WANG J.Y., JIANG W.S., LIU D.H. Cadmium localization and its toxic effects on root tips of barley. Zemdirbyste 103, 151, 2016.
• 5. ZOU J.H., YUE J.Y., JIANG W.S., LIU D.H. Effects of cadmium stress on root tip cells and some physiological indexes in Allium cepa var. agrogarum L. Acta Biol. Cracov. Bot. 54, 129, 2012.
• 6. QIN R., JIANG W.S., LIU D.H. Cadmium can induce alterations in the cellular localization and expression of three major nucleolar proteins in root tip cells of Vicia faba L. Plant Soil 368, 365, 2013.
• 7. ZHANG H.H., JIANG Z., QIN R., ZHANG H.N., ZOU J.H., JIANG W.S., LIU D.H. Accumulation and cellular toxicity of aluminum in seedling of Pinus massoniana. BMC Plant Biol. 14, 264, 2014.
• 8. LIU D.H., KOTTKE I. Subcellular localization of cadmium in the root cells of Allium cepa by electron energy loss spectroscopy and cytochemistry. J. Biosciences 29, 329, 2004.
• 9. JIANG W.S., LIU D.H., XU P. Cd-induced system of defence in the garlic root meristematic cells. Biol. Plantarum 53, 369, 2009.
• 10. GE W., JIAO Y.Q., SUN B.L., QIN R., JIANG W.S., LIU D.H. Cadmium-mediated oxidative stress and ultrastructural changes in root cells of poplar cultivars. S. Afr. J. Bot. 83, 98, 2012.
• 11. JIAO Y.Q., GE W., QIN R., SUN B.L., JIANG W.S., LIU D.H. Influence of cadmium stress on growth, ultrastructure and antioxidative enzymes in Populus 2025. Fresen. Environ. Bull. 21, 1375, 2012.
• 12. LIU X.J., SHI Q.Y., ZOU J.H., WANG J.R., WU H.F., WANG J.Y., JIANG W.S., LIU D.H. Chromosome and nucleolus morphological characteristics in root tip cells of plants under metal stress. Fresen. Environ. Bull. 25, 2419, 2016.
• 13. ADAMAKIS I.D.S., PANTERIS E., CHERIANIDOU A., ELEFTHERIOU E.P. Effects of bisphenol A on the microtubule arrays in root meristematic cells of Pisum sativum L. Mutat. Res-Gen. Tox. En. 750, 111, 2013.
• 14. BAGNIEWSKA-ZADWORNA A. The root microtubule cytoskeleton and cell cycle analysis through desiccation of Brassica napus seedlings. Protoplasma 233, 177, 2008.
• 15. GZYL J., CHMIELOWSKA-BĄK J., PRZYMUSIŃSKI R., GWÓŹDŹ E.A. Cadmium affects microtubule organization and post-translational modifications of tubulin in seedlings of soybean (Glycine max L.). Front. Plant Sci. 6, 937, 2015.
• 16. PARROTTA L., CRESTI M., CAI G. Accumulation and post-translational modifications of plant tubulins. Plant Biology 16, 521, 2014.
• 17. BREVIARIO D., GIANÌ S., MORELLO L. Multiple tubulins: evolutionary aspects and biological implications. Plant J. 75, 202, 2013.
• 18. YU Y.L., LI Y.Z., LI L.L., LIN J.X., ZHENG C.C., ZHANG L.Y. Overexpression of PwTUA1, a pollen-specific tubulin gene, increases pollen tube elongation by altering the distribution of a-tubulin and promoting vesicle transport. J. Exp. Bot. 60, 2737, 2009.
• 19. OAKLEY R.V., WANG Y.S., RAMAKRISHNA W., HARDING S.A., TSAI C.J. Differential expansion and expression of α- and β-tubulin gene families in Populus. Plant Physiol. 145, 961, 2007.
• 20. SUI J.K., RAO G.D., ZHANG J.G. Cloning and sequence analysis of α-tubulin gene families in Salix matsudana Koidz and S. matsudana var. tortuosa (Vilm.) Rehd. Mol Plant Breeding 14, 2305, 2016.
• 21. LING T., JUN R., FANGKE Y. Effect of cadmium supply levels to cadmium accumulation by Salix. Int. J. Environ. Sci. Technol. 8, 493, 2011.
• 22. YANG J.L., LI K., ZHENG W., ZHANG H.Z., CAO X.D., LAN Y.X., YANG C.P., LI C.H. Characterization of early transcriptional responses to cadmium in the root and leaf of Cd-resistant Salix matsudana Koidz. BMC Genomics 16, 705, 2015.
• 23. ZOU J.H., WANG G., JI J., WANG J.Y., WU H.F., OU Y.J., LI B.B. Transcriptional, physiological and cytological analysis validated the roles of some key genes linked Cd stress in Salix matsudana Koidz. Environ. Exp. Bot. 134, 116, 2017.
• 24. DICKINSON N.M., PULFORD I.D. Cadmium phytoextraction using short-rotation coppice Salix: the evidence trail. Environ. Int. 31, 609, 2005.
• 25. DOS SANTOS UTMAZIAN M.N., WIESHAMME G., VEGA R., WENZEL W.W. Hydroponic screening for metal resistance and accumulation of cadmium and zinc in twenty clones of willows and poplars. Environ. Pollut. 148, 155, 2007.
• 26. KACÁLKOVÁ L., TLUSTOŠ P., SZÁKOVÁ J. Phytoextraction of risk elements by willow and poplar trees. Int. J. Phytoremediat. 17, 414, 2015.
• 27. YANG J.L., YI J., YANG C.P., LI C.H. Agrobacterium tumefaciens-mediated genetic transformation of Salix matsudana Koidz. using mature seeds. Tree Physiol. 33, 628, 2013.
• 28. WU H.F., WANG J.Y., OU Y.J., LI B.B., JIANG W.S., LIU D.H., ZOU J.H. Cadmium uptake and localization in roots of Salix matsudana Koidz. Fresen. Environ. Bull. 25, 2700, 2016.
• 29. WAN X.M., LEI M., YANG J.X. Two potential multi-metal hyperaccumulators found in four mining sites in Hunan Province, China. Catena. 148, 67, 2017.
• 30. LIAO T.T., SHI Y.L., JIA J.W., WANG L. Sensitivity of different cytotoxic responses of Vero cells exposed to organic chemical pollutants and their reliability in the bio-toxicity test of trace chemical pollutants. Biomed. Environ. Sci. 23, 219, 2010.
• 31. WANG J.R., SHI Q.Y., ZOU J.H., JIANG Z., WANG J.Y., WU H.F., JIANG W.S., LIU D.H. Cellular localization of copper and its toxicity on root tips of Hordeum vulgare. Fresen. Environ. Bull. 24, 2394, 2015.
• 32. GARDINER J., OVERALL R., MARC J. Plant microtubule cytoskeleton complexity: microtubule arrays as fractals. J. Exp. Bot. 63, 635, 2012.
• 33. ELEFTHERIOU E.P., ADAMAKIS I.S., MELISSA P. Effects of hexavalent chromium on microtubule organization, ER distribution and callose deposition in root tip cells of Allium cepa L. Protoplasma 249, 401, 2012.
• 34. XU P., LIU D.H., JIANG W.S. Cadmium effects on the organization of microtubular cytoskeleton in interphase and mitotic cells of Allium sativum. Biol. Plant. 53, 387, 2009.
• 35. LIU D.H., XUE P., MENG Q.M., ZOU J., GU J.G., JIANG W.S. Pb/Cu effects on the organization of microtubule cytoskeleton in interphase and mitotic cells of Allium sativum L. Plant Cell. Rep. 28, 695, 2009.
• 36. RASMUSSEN C.G., WRIGHT A.J., MÜLLER S. The role of the cytoskeleton and associated proteins in determination of the plant cell division plane. Plant J. 75, 258, 2013.
• 37. RAO G.D., ZENG Y.F., HE C.Y., ZHANG J.G. Characterization and putative posttranslational regulation of α- and β-tubulin gene families in Salix arbutifolia. Sci. Rep. 6, 19258, 2016.
• 38. LIVAK K.J., SCHMITTGEN T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25, 402, 2001.

Identyfikatory

DOI

10.15244/pjoes/76031