Identification of candidate genes associated with male sterility in CMS7311 of heading Chinese cabbage (Brassica campestris L. ssp. pekinensis)

• Autorzy: Xu X. , Sun X. , Zhang J. , Huang W. , Zhang L. , Fang Z.
• Języki publikacji: EN

Abstrakty

EN

Polima (pol) cytoplasmic male sterility (CMS) is reported to be regulated by orf224, but the molecular mechanisms of CMS are less known. In previous research, CMS7311 of heading Chinese cabbage (Brassica campestris L.ssp. pekinensis) was successfully generated from a pol-like CMS, named 77A. To further understand the molecular mechanisms of this male sterility, cDNA-AFLP and qRT-PCR were used to identify genes differentially expressed during flower bud development between cytoplasmic male-sterile plant (Ms) and male-fertile plant (Mf) derived from the hybrid of CMS7311 and a restorer (Rf). Thirty-two transcripts of different fragments (TDFs) of over 80 bp in length were identified using cDNA-AFLP. Among them, ten TDFs were found expressing in Ms bud, and the others expressing in Mf bud. Gene ontology analysis revealed that these genes were involved in stress response, synthesis and metabolism of fatty acid, cell reconstruction, etc. Further, five genes closely related to male sterile were selected and their expression during the flower bud development of Ms and Mf line were investigated by qRT-PCR. Three genes, BcGRP17, BcMS2, and BcPME31, which were necessary for normal male organ development, showed significantly lower expressions in Ms bud; two genes, BcROPGEF8 and BcTNL3, were induced with significantly higher expression in Ms bud by unknown stress and involved in the formation of male sterility. Further work will be focused on cloning these genes and identifying their functions in the male-sterile process of heading Chinese cabbage CMS7311.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 11
• Opis fizyczny: p.3265-3270,fig.,ref.

Twórcy

autor

Xu X.

• State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi, People’s Republic of China
• College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People’s Republic of China

autor

Sun X.

• State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi, People’s Republic of China
• College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People’s Republic of China

autor

Zhang J.

• State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi, People’s Republic of China
• College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People’s Republic of China

autor

Huang W.

• State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi, People’s Republic of China

autor

Zhang L.

• State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, Shaanxi, People’s Republic of China
• College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People’s Republic of China

autor

Fang Z.

• College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, People’s Republic of China

Bibliografia

• Aarts MGM, Hodge R, Kalantidis K, Florack D, Wilson ZA, Mulligan BJ, Stiekema WJ, Scott R, Pereira A (1997) The Arabidopsis MALE STERILITY 2 protein shares similarity with reductases in elongation/condensation complexes. Plant Journal 12(3):615–623
• Andersen CL, Jensen JL, Orntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64(15):5245–5250. doi:10.1158/0008-5472.can-04-0496
• Belkhadir Y, Subramaniam R, Dangl JL (2004) Plant disease resistance protein signaling: NBS-LRR proteins and their partners. Curr Opin Plant Biol 7(4):391–399. doi:10.1016/j.pbi.2004.05.009
• Berken A, Thomas C, Wittinghofer A (2005) A new family of RhoGEFs activates the Rop molecular switch in plants. Nature 436(7054):1176–1180. doi:10.1038/nature03883
• Chase CD (2007) Cytoplasmic male sterility: a window to the world of plant mitochondrial–nuclear interactions. Trends Genet 23(2):81–90. doi:10.1016/j.tig.2006.12.004
• Chen W, Yu XH, Zhang K, Shi J, De Oliveira S, Schreiber L, Shanklin J, Zhang D (2011) Male Sterile2 encodes a plastidlocalized fatty acyl carrier protein reductase required for pollen exine development in Arabidopsis. Plant Physiol 157(2):842–853. doi:10.1104/pp.111.181693
• Cheng F, Liu S, Wu J, Fang L, Sun S, Liu B, Li P, Hua W, Wang X (2011) BRAD, the genetics and genomics database for Brassica plants. BMC Plant Biol 11:136. doi:10.1186/1471-2229-11-136
• de Oliveira DE, Franco LO, Simoens C, Seurinck J, Coppieters J, Botterman J, Van Montagu M (1993) Inflorescence-specific genes from Arabidopsis thaliana encoding glycine-rich proteins. Plant J 3(4):495–507
• Elmore JM, Lin ZJ, Coaker G (2011) Plant NB-LRR signaling: upstreams and downstreams. Curr Opin Plant Biol 14(4):365–371. doi:10.1016/j.pbi.2011.03.011
• Fujii S, Komatsu S, Toriyama K (2007) Retrograde regulation of nuclear gene expression in CW-CMS of rice. Plant Mol Biol 63(3):405–417. doi:10.1007/s11103-006-9097-8
• Geddy R, Mahe L, Brown GG (2005) Cell-specific regulation of a Brassica napus CMS-associated gene by a nuclear restorer with related effects on a floral homeotic gene promoter. Plant J 41(3):333–345. doi:10.1111/j.1365-313X.2004.02305.x
• Jiang M, Cao JS (2008) Isolation and characterization of a male sterility gene homolog BcMS2 from Chinese cabbagge-pak-choi that expressing in an anther-specific manner. Mol Biol Rep 35(3):299–305. doi:10.1007/s11033-007-9086-2
• Liu LC, Yu XL, Ye WZ, Xiang X, Cao JS (2006) Functional confirmation of BcMF3 gene related to male sterility of Chinese cabbage by antisense RNA. J Zhejiang Univ (Agric Life Sci) 32(05):473–478 (in Chinese)
• Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4):402–408. doi:10.1006/meth.2001.1262
• Mayfield JA, Preuss D (2000) Rapid initiation of Arabidopsis pollination requires the oleosin-domain protein GRP17. Nat Cell Biol 2(2):128–130. doi:10.1038/35000084
• Mun JH, Yu HJ, Park S, Park BS (2009) Genome-wide identification of NBS-encoding resistance genes in Brassica rapa. Mol Genet Genomics 282(6):617–631. doi:10.1007/s00438-009-0492-0
• Shin D, Kim T-L, Kwon Y-K, Cho M-H, Yoo J, Jeon J-S, Hahn T-R, Bhoo S (2009) Characterization of Arabidopsis RopGEF family genes in response to abiotic stresses. Plant Biotechnology Reports 3(3):183–190. doi:10.1007/s11816-009-0090-y
• Singh M, Brown GG (1991) Suppression of cytoplasmic male-sterility by nuclear genes alters expression of a novel mitochondrial gene region. Plant Cell 3(12):1349–1362
• Singh M, Brown GG (1993) Characterization of expression of a mitochondrial gene region associated with the Brassica ‘‘Polima’’ CMS: developmental influences. Curr Genet 24(4):316–322
• Tian GW, Chen MH, Zaltsman A, Citovsky V (2006) Pollen-specific pectin methylesterase involved in pollen tube growth. Dev Biol 294(1):83–91. doi:10.1016/j.ydbio.2006.02.026
• Wan C, Li S, Wen L, Kong J, Wang K, Zhu Y (2007) Damage of oxidative stress on mitochondria during microspores development in Honglian CMS line of rice. Plant Cell Rep 26(3):373–382. doi:10.1007/s00299-006-0234-2
• Wang YQ, Yu XL, Cao JS (2004) Isolation and characterization of BcMF3, a gene expressed only in maintainer line in Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino var. communis Tsen et lee). Yi Chuan Xue Bao 31(11):1302–1308 (in Chinese)
• Zhang LG, Hao DF (2001) Investigation on the sterility changeover of male sterility line CMS7311 in heading Chinese cabbage. Acta Bot Sinica 43(11):1123–1128
• Zhang ZF, Zhang LG, Wang Q, Hui MX, Zhang MK (2006) RNA fingerprinting of the differential expression fragments related to cytoplasmic male sterility in Chinese cabbage. Yi Chuan 28(10):1280–1286. doi:0253-9772(2006)10-1280-07(InChinese
• Zhang CW, Qi L, Hou XL, Shi GJ, Zhang JY (2010) Differential gene expression analysis of a new Ogura CMS line and its maintainer in non-heading Chinese cabbage by cDNA-AFLP. Acta Physiologiae Plantarum 32(4):781–787. doi:10.1007/s11738-010-0463-4

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