Effects of pattern deprivation on the development of the motion-sensitive posterior medial lateral suprasylvian (PMLS) visual area of the cat as revealed by zif268 gene expression

• Autorzy: Zapasnik M. , Laskowska-Macios K. , Hu T.T. , Kossut M. , Arckens L. , Burnat K.
• Języki publikacji: EN

Abstrakty

EN

Long-term binocular pattern deprivation (BD) from eye opening results in severe global motion perception impairment in children and cats. We recently showed in cat that late onset deprivation (month 3 and 4 following 2 months of normal vision; 2N2BD), also leads to global motion perception impairments. Interestingly, 2 months of BD from eye opening facilitates motion perception, while a continuous 4 month BD period from birth (4BD) does not result in significant impairment. To test how these BD outcomes are reflected at the level of neuronal activity we compared the expression profile of the activity reporter gene zif268 in PMLS in three separate BD conditions. Adult and juvenile 4BD, 6BD and 2N2BD cats and age-matched controls were used. Cats were exposed to overnight darkness prior to 1-hour light stimulation and then sacrificed; kittens at the end of the BD period, adults after 2 years of visual training upon BD. In situ hybridization was applied using an oligonucleotide probe complementary to the nucleotides encoding amino acids 2-16 of the rat zif268 gene. For 4BD and 2N2BD kittens the zif268 mRNA level in PMLS was significantly elevated by 50%, as compared to age-matched controls. 6BD kittens also had elevated zif268 signals, but by 21%. Interestingly, zif268 expression in PMLS increases with age as in control kittens higher levels were observed after 6 than 4 months of age. In adult cats zif268 signals were highest in the 4BD group, while 2N2BD cats did not differ from controls. We infer that the developmental pattern of the motionsensitive area PMLS is affected by early BD. BD most likely prolongs the processes of cortical development, as zif268 mRNA levels in 4BD and 2N2BD kittens are equally high. Surprisingly, restoration of normal visual input in adult 2N2BD cats leads to normalization of activity levels in PMLS, while in the 4BD group it remains high. These results link high zif268 gene expression to behavioral performance.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.41

Twórcy

autor

Zapasnik M.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Laskowska-Macios K.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Hu T.T.

• Neuroplasticity and Neuroproteomics Unit, K.U.Leuven, Leuven, Belgium

autor

Kossut M.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

autor

Arckens L.

• Neuroplasticity and Neuroproteomics Unit, K.U.Leuven, Leuven, Belgium

autor

Burnat K.

• Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology PAS, Warsaw, Poland

Uwagi

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