Life in extreme habitats: diversity of endolithic microorganisms from cold desert ecosystems of Eastern Pamir

• Autorzy: Khomutovska N. , Jerzak M. , Kostrzewska-Szlakowska I. , Kwiatkowski J. , Suska-Malawska M. , Syczewski M. , Jasser I.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to identify cyanobacteria diversity in rock communities from the cold desert ecosystem in Eastern Pamir Mountains (Tajikistan) and assess if the rock type and rock`s porosity can be indicators of microbial diversity in this extreme environment. Seven samples were collected in July 2015 from hillsides (ca 4000–4500 m a.s.l.) of the Eastern Pamir Mountains. Petrographic and scanning microscopy (SEM) allowed for the characterization of the rocks inhabited by endolithic communities as granite, gneiss and limestone with variable porosity. Based on next-generation sequencing (NGS) of amplicon of V3–V4 hypervariable region of 16S rRNA gene, we established that Actinobacteria,Proteobacteria and Cyanobacteria dominated the endolithic communities of microorganisms in the rocks studied, which distinguishes these communities from those described for other cold arid regions. Chroococcidiopsis and Leptolyngbya were dominant genera in the cyanobacterial communities according to culture-dependent analysis, as well as microscopic analyses of endoliths scraps from the rocks. Culture-independent metagenomic analyses revealed that Microcoleus, Acaryochloris, Chroococcidiopsis and Thermosynechococcus reads were the most abundant from all reads and dominated interchangeably in the samples. Endolithic communities of microorganisms in the rocks from the cold desert shrubland of Eastern Pamir Mts. appear to be diverse and different from communities described for other cold deserts.

Słowa kluczowe

EN

desert ecosystem; 16S rDNA; metagenomics; Cyanoprokaryota; Proteobacteria; Actinobacteria; microbial diversity; Pamir Mountains; Tajikistan

• Wydawca: -
• Czasopismo: Polish Journal of Ecology
• Rocznik: 2017
• Tom: 65
• Numer: 4
• Opis fizyczny: p.303-319,fig.,ref.

Twórcy

autor

Khomutovska N.

• Department of Plant Ecology and Environmental Conservation, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02−089 Warsaw, Poland

autor

Jerzak M.

• Department of Plant Ecology and Environmental Conservation, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02−089 Warsaw, Poland

autor

Kostrzewska-Szlakowska I.

• Faculty of Biology, University of Warsaw, Miecznikowa 1, 02−096, Warsaw, Poland

autor

Kwiatkowski J.

• Department of Molecular Phylogenetics and Evolution, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02−089 Warsaw, Poland

autor

Suska-Malawska M.

• Department of Plant Ecology and Environmental Conservation, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02−089 Warsaw, Poland

autor

Syczewski M.

• Institute of Geochemistry, Mineralogy and Petrology, Faculty of Geology, University of Warsaw, Zwirki i Wigury 93, 02−089 Warsaw, Poland

autor

Jasser I.

• Department of Plant Ecology and Environmental Conservation, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02−089 Warsaw, Poland

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Identyfikatory

DOI

10.3161/15052249PJE2017.65.4.001