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2012 | 56 | 3 |
Tytuł artykułu

Monitoring of selected genes in Campylobacter jejuni and Campylobacter coli isolates from domestic animals

Autorzy
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In the present study, the presence of flaA, cadF, cdtB, and iam genes of Campylobacter sp. were analysed using PCR. Material for analyses comprised 100 Campylobacter sp. isolates obtained from healthy broiler chickens, fatteners, and calves, among which 84 isolates were ascribed to Campylobacter jejeuni and 16 to Campylobacter coli. All isolates (100%) had the cadF gene responsible for adhesion and the flaA gene determining the motility of the analysed bacteria. The frequency of occurrence of the cdtB gene responsible for the production of the cytolethal distending toxin (CDT) was determined to be high (98.6% in broiler chickens, 75% in fatteners, 62.5% in calves). In case of the iam gene, the highest frequency was recorded in Campylobacter sp. isolated from broiler chickens (84.7%), while in strains collected from fatteners and calves it was lower, amounting to 41.7% and 18.8%, respectively.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
56
Numer
3
Opis fizyczny
p.283-286,fig.,ref.
Twórcy
autor
  • Department of General and Environmental Biology, Poznan University of Life Sciences, 60-656 Poznan, Poland
autor
Bibliografia
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  • 2. Bang D.D., Scheutz F., Ahrens P., Pedersen K., Blom J., Madsen M.: Prevalence of cytolethal distending toxin (cdt) genes and CDT production in Campylobacter spp. isolated from Danish broilers. J Med Microbiol 2001, 50, 1087-1094.
  • 3. Carvalho A.C., Ruiz-Palacios G.M., Ramos-Cervantes P., Cervantes L.E., Jiang X., Pickering L.K.: Molecular characterization of invasive and noninvasive Campylobacter jejuni and Campylobacter coli isolates. J Clin Microbiol 2001, 39, 1353-1359.
  • 4. European Food Safety Authority and European Centre for Disease Prevention and Control; the European Union Summary Report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2010. EFSA J 2012, 10, 233.
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  • 6. Heywood W., Henderson B., Nair S.P.: Cytolethal distending toxin: creating a gap in the cell cycle. J Med Microbiol 2005, 54, 207-216.
  • 7. Jang K.I., Kim M.G., Ha S.D., Kim K.S., Lee K.H., Chung D.H., Kim C.H., Kim K.Y.: Morphology and adhesion of Campylobacter jejuni to chicken skin under varying conditions. J Microbiol Biotechnol 2007, 17, 202-206.
  • 8. Konkel M., Gray S.A., Kim B.J., Gravis S.G., Yoon J.: Identification of enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its product. J Clin Microbiol 1999, 37, 510- 517.
  • 9. Krutkiewicz A.: Campylobacteriosis in humans and animals. Życie Wet 2008, 4. 285-288.
  • 10. Lara-Tejero M., Galan J.E.: Cdt A, cdtB and cdtC form a tripartite complex that is required for cytolethal distending toxin activity. Infect Immun 2001, 69, 4358- 4365.
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  • 14. Pejsak Z., Truszczyński M.: Diarrhoeal diseases in swine. Życie Wet 2007, 82, 108-110.
  • 15. Rizal A., Kumar A., Vidyarthi A.S.: Prevalence of pathogenic genes in Campylobacter jejuni isolated from poultry and human. Internat J Food Safety 2010, 12, 1531-1536.
  • 16. Rozynek E., Dzierzanowska-Fangrat K., Jozwiak P., Popowski J., Korsak D., Dzierzanowska D.: Prevalence of potential virulence markers in Polish Campylobacter jejuni and Campylobacter coli isolates obtained from hospitalized children and from chicken carcasses. J Med Microbiol 2005, 54, 615-619.
  • 17. Rzedzicki J., Kołodziejczyk A.: Birds as a potential source of human infection by Campylobacter sp. Medycyna Wet 1998, 54, 511 -514
  • 18. Talukder K.A., Aslam M., Islam Z., Azmi I.J., Dutta D.K., Hossain S., Nur-E-Kamal A., Nair G.B., Cravioto A., Sack D.A., Endtz H.P.: Prevalence of virulence genes and cytolethal distending toxin in Campylobacter jejuni isolates from diarrheal patients. J Clin Microbiol 2008, 46, 1485-1488.
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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