Identification and expression analysis of Rehmannia glutinosa mediator complex genes in response to continuous cropping

• Autorzy: Wang F. , Tian Y. , Wei H. , Suo Y. , Xie C. , Li M. , Huang Y. , Chen X. , Zhang Z.
• Języki publikacji: EN

Abstrakty

EN

The mediator complex, a conserved assembly of multi-functional proteins, is required for the transcription of almost all protein-encoding genes in eukaryotes. In this report, 36 mediator complex subunit transcripts in Rehmannia glutinosa were identified from a leaf and tuberous root EST database. Nine of these transcripts had integrated open-reading frames (ORFs). A comparison of predicted amino acid sequences with putative mediator complex subunit homologues from another six flowering plants revealed high identities and several conserved domains. Phylogenetic analysis of the nine mediator complex genes in seven flowering plants revealed a closer evolutionary relationship between R. glutinosa and Solanum lycopersicum. The relative abundances of the 36 transcripts were analysed in the leaves and tuberous roots of R. glutinosa. QRT-PCR analysis revealed that the transcripts of approximately one-third of the mediator complex genes investigated in this study were expressed at similar levels. This result suggested that these transcripts might contribute to the formation, maturation or functions of the analysed tissues. The effects of consecutive monoculture on the transcription of R. glutinosa mediator complex genes were also analysed by qRT-PCR. This analysis revealed that onethird of the mediator complex genes presented differential expression patterns in the leaves of second-year plants, whereas over half of the mediator genes were differentially expressed in the tuberous roots of second-year plants. These patterns suggest that one of the subunits of the mediator complex regulates the consecutive monoculture problem in R. glutinosa.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 12
• Opis fizyczny: Article: 264 [13 p.], fig.,ref.

Twórcy

autor

Wang F.

• College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China

autor

Tian Y.

• College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China
• College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

autor

Wei H.

• Institute of Industrial Crops, Henan Academy of Agricultural Science, Zhengzhou, 450002, China

autor

Suo Y.

• College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China

autor

Xie C.

• School of Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, China

autor

Li M.

• College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

autor

Huang Y.

• College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China

autor

Chen X.

• College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China

autor

Zhang Z.

• College of Crop Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

Bibliografia

• Alagna F, Mariotti R, Panara F, Caporali S, Urbani S, Veneziani G, Esposto S, Taticchi A, Rosati A, Rao R, Perrotta G, Servili M, Baldoni L (2012) Olive phenolic compounds: metabolic and transcriptional profiling during fruit development. BMC Plant Biol 12:162
• Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J (2002) Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene. EMBO J 21:6036–6049
• Bäckström S, Elfving N, Nilsson R, Wingsle G, Björklund S (2007) Purification of a plant mediator from Arabidopsis thaliana identifies PFT1 as the Med25 subunit. Mol Cell 26:717–729
• Björklund S, Gustafsson CM (2005) The yeast mediator complex and its regulation. Trends Biochem Sci 30:240–244
• Bonawitz ND, Soltau WL, Blatchley MR, Powers BL, Hurlock AK, Seals LA, Weng JK, Stout J, Chapple C (2012) REF4 and RFR1, subunits of the transcriptional coregulatory complex mediator, are required for phenylpropanoid homeostasis in Arabidopsis. J Biol Chem 287:5434–5445
• Borggrefe T, Yue X (2011) Interactions between subunits of the mediator complex with gene-specific transcription factors. Semin Cell Dev Biol 22:759–768
• Boube M, Faucher C, Joulia L, Cribbs DL, Bourbon HM (2000) Drosophila homologs of transcriptional mediator complex subunits are required for adult cell and segment identity specification. Genes Dev 14:2906–2917
• Boube M, Joulia L, Cribbs DL, Bourbon HM (2002) Evidence for a mediator of RNA polymerase II transcriptional regulation conserved from yeast to man. Cell 110:143–151
• Bourbon HM (2008) Comparative genomics supports a deep evolutionary origin for the large, four-module transcriptional mediator complex. Nucleic Acids Res 36:3993–4008
• Bourbon HM, Aguilera A, Ansari AZ, Asturias FJ, Berk AJ, Bjorklund S, Blackwell TK, Borggrefe T, Carey M, Carlson M, Conaway JW, Conaway RC, Emmons SW, Fondell JD, Freedman LP, Fukasawa T, Gustafsson CM, Han M, He X, Herman PK, Hinnebusch AG, Holmberg S, Holstege FC, Jaehning JA, Kim YJ, Kuras L, Leutz A, Lis JT, Meisterernest M, Naar AM, Nasmyth K, Parvin JD, Ptashne M, Reinberg D, Ronne H, Sadowski I, Sakurai H, Sipiczki M, Sternberg PW, Stillman DJ, Strich R, Struhl K, Svejstrup JQ, Tuck S, Winston F, Roeder RG, Kornberg RD (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14:553–557
• Cai G, Imasaki T, Takagi Y, Asturias FJ (2009) mediator structural conservation and implications for the regulation mechanism. Structure 17:559–567
• Dhawan R, Luo H, Foerster AM, Abuqamar S, Du HN, Briggs SD, Mittelsten Scheid O, Mengiste T (2009) HISTONE MONOUBIQUITINATION1 interacts with a subunit of the mediator complex and regulates defense against necrotrophic fungal pathogens in Arabidopsis. Plant Cell 21:1000–1019
• Gill G, Ptashne M (1988) Negative effect of the transcriptional activator GAL4. Nature 334:721–724
• Gillmor C, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS (2014) The Arabidopsis mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions. Development 141:4580–4589
• Gu W, Malik S, Ito M, Yuan CX, Fondell JD, Zhang X, Martinez E, Qin J, Roeder RG (1999) A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation. Mol Cell 3:97–108
• Guo GY, Li MJ, Wang PF, Wang FQ, He HQ, Li J, Zheng HY, Chen XJ, Zhang ZY (2013) Abnormal change of calcium signal system on continuous cropping obstacle of Rehmannia glutinosa. China J Chin Mater Med 38:1471–1478
• Hemsley PA, Hurst CH, Kaliyadasa E, Lamb R, Knight MR, De Cothi EA, Steele JF, Knight H (2014) The Arabidopsis mediator complex subunits MED16, MED14, and MED2 regulate mediator and RNA polymerase II recruitment to CBF-responsive cold-regulated genes. Plant Cell 26:465–484
• Imura Y, Kobayashi Y, Yamamoto S, Furutani M, Tasaka M, Abe M, Araki T (2012) CRYPTIC PRECOCIOUS/MED12 is a novel flowering regulator with multiple target steps in Arabidopsis. Plant Cell Physiol 53:287–303
• Ito J, Sono T, Tasaka M, Furutani M (2011) MACCHI-BOU 2 is required for early embryo patterning and cotyledon organogenesis in Arabidopsis. Plant Cell Physiol 52:539–552
• Jiang YW, Veschambre P, Erdjument-Bromage H, Tempst P, Conaway JW, Conaway RC, Kornberg RD (1998) Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways. Proc Natl Acad Sci USA 95:8538–8543
• Kidd BN, Edgar CI, Kumar KK, Aitken EA, Schenk PM, Manners JM, Kazan K (2009) The mediator complex subunit PFT1 is a key regulator of jasmonate-dependent defense in Arabidopsis. Plant Cell 21:2237–2252
• Kidd BN, Cahill DM, Manners JM, Schenk PM, Kazan K (2011) Diverse roles of the mediator complex in plants. Semin Cell Dev Biol 22:741–748
• Knight H, Veale EL, Warren GJ, Knight MR (1999) The sfr6 mutation in Arabidopsis suppresses low-temperature induction of genes dependent on the CRT/DRE sequence motif. Plant Cell 11:875–886
• Knight H, Thomson AJW, McWatters HG (2008) Sensitive to freezing6 integrates cellular and environmental inputs to the plant circadian clock. Plant Physiol 148:293–303
• Knight H, Mugford SG, Ulker B, Gao D, Thorlby G, Knight MR (2009) Identification of SFR6, a key component in cold acclimation acting post-translationally on CBF function. Plant J 58:97–108
• Kornberg RD (2007) The molecular basis of eukaryotic transcription. Proc Natl Acad Sci USA 104:12955–12961
• Li MJ, Yang YH, Chen XJ, Wang FQ, Lin WX, Yi YJ, Zeng L, Yang SY, Zhang ZY (2013) Transcriptome/degradome-wide identification of R. glutinosa miRNAs and their targets: the role of mirna activity in the replanting disease. PLoS One 8:e68531
• Liu YF, Liang D, Luo H, Hao ZY, Wang Y, Zhang CL, Zhang QJ, Chen RY, Yu DQ (2012) Hepatoprotective iridoid glycosides from the roots of Rehmannia glutinosa. J Nat Prod 75:1625–1631
• Malik S, Roeder RG (2010) The metazoan mediator co-activator complex as an integrative hub for transcriptional regulation. Nat Rev Genet 11:761–772
• Mathur S, Vyas S, Kapoor S, Tyagi AK (2011) The mediator complex in plants: structure, phylogeny, and expression profiling of representative genes in a dicot (Arabidopsis) and a monocot (rice) during reproduction and abiotic stress. Plant Physiol 157:1609–1627
• McGrath KC, Dombrecht B, Manners JM, Schenk PM, Edgar CI, Maclean DJ, Scheible WR, Udvardi MK, Kazan K (2005) Repressor-and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression. Plant Physiol 139:949–959
• Niu M, Fan H, Li J, Du J, Chen X, Zhang Z (2011) Effects of leaf removal on growth and physiological characteristics of Rehmannia glutinosa. China J Chin Mater Med 36:107–111
• Pasrija R, Thakur JK (2013) Tissue specific expression profile of mediator genes in Arabidopsis. Plant Signal Behav 8:e23983
• Poss ZC, Ebmeier CC, Taatjes DJ (2013) The mediator complex and transcription regulation. Crit Rev Biochem Mol Biol 48:575–608
• Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3:1101–1108
• Stout J, Romero-Severson E, Ruegger MO, Chapple C (2008) Semidominant mutations in reduced epidermal fluorescence 4 reduce phenylpropanoid content in Arabidopsis. Genetics 178:2237–2251
• Sun P, Xiao X, Duan L, Guo Y, Qi J, Liao D, Zhao C, Liu Y, Li X (2015) Dynamic transcriptional profiling provides insights into tuberous root development in Rehmannia glutinosa[J]. Front Plant Sci 6:396–409
• Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739
• Wang F, Wei H, Tong Z, Zhang X, Yang Z, Lan T, Duan Y, Wu W (2011) Knockdown of NtMed8, a Med8-like gene, causes abnormal development of vegetative and floral organs in tobacco (Nicotiana tabacum L.). Plant Cell Rep 30:2117–2129
• Wang FQ, Tian YH, Wei H, Gu L, Wang JM, Zhang ZY (2012) The mediator complex in plant: component, function and expression profile. Acta Bot Boreal -occident Sin 32:2124–2132
• Wathugala DL, Richards SA, Knight H, Knight MR (2011) OsSFR6 is a functional rice orthologue of SENSITIVE TO FREEZING-6 and can act as a regulator of COR gene expression, osmotic stress and freezing tolerance in Arabidopsis. New Phytol 191:984–995
• Wu L, Wang H, Zhang Z, Lin R, Zhang Z, LinW (2011) Comparative metaproteomic analysis on consecutively Rehmannia glutinosamonocultured rhizosphere soil. PLoS ONE 6:e20611
• Wu LK, Li ZF, Li J, Khan MA, Huang WM, Zhang ZY, Lin WX (2013) Assessment of shifts in microbial community structure and catabolic diversity in response to Rehmannia glutinosa monoculture. Appl Soil Ecol 67:1–9
• Xu R, Li Y (2011) Control of final organ size by mediator complex subunit 25 in Arabidopsis thaliana. Development 138:4545–4554
• Xu R, Li Y (2012) The mediator complex subunit 8 regulates organ size in Arabidopsis thaliana. Plant Signal Behave 7:182–183
• Yang YH, Chen XJ, Chen JY, Xu HX, Li J, Zhang ZY (2011) Differential miRNA expression in Rehmannia glutinosa plants subjected to continuous cropping. BMC Plant Biol 11:53
• Zhang X, Yao J, Zhang Y, Sun Y, Mou Z (2013) The Arabidopsis mediator complex subunits MED14/SWP and MED16/SFR6/IEN1 differentially regulate defense gene expression in plant immune responses. Plant J 75:484–497
• Zheng Z, Guan H, Leal F, Grey PH, Oppenheimer DG (2013) mediator subunit18 controls flowering time and floral organ identity in Arabidopsis. PLoS One 8:e53924
• Zhu Y, Schluttenhoffer CM, Wang P, Fu F, Thimmapuram J, Zhu JK, Lee SY, Yun DJ, Mengiste T (2014) CYCLIN-DEPENDENT KINASE8 differentially regulates plant immunity to fungal pathogens through kinase-dependent and-independent functions in Arabidopsis. Plant Cell 26:4149–4170

Identyfikatory

DOI

10.1007/s11738-015-2020-7