A protocol for sonication - assisted Agrobacterium rhizogenesmediated transformation of haploid and diploid sugar beet (Beta vulgaris L.) explants

• Autorzy: Klimek-Chodacka M. , Baranski R.
• Języki publikacji: EN

Abstrakty

EN

 Hairy root cultures obtained after Agrobacterium rhizogenes-mediated genetic transformation can serve as a model system for studying plant metabolism and physiology, or can be utilized for the production of secondary metabolites. So far no efficient protocol of hairy root development in sugar beet has been publically released. In this work, two A. rhizogenes strains (A4T and LBA1334) carrying a binary vector pBIN-m-gfp5-ER or pCAMBIA1301 possessing gfp and uidA reporter genes were used to transform petiole explants of haploid and diploid sugar beet genotypes. Five treatment combinations of sonicated-assisted Agrobacterium-mediated transformation were compared. Hairy roots appeared on 0% to 54% of explants depending on the treatment combination used. The highest frequency was achieved when explants of a diploid genotype were sonicated for 15 s in the inoculum containing A. rhizogenes of OD600=0.5 and then co-cultured for three days. Using the same treatment combinations the explants of haploid genotypes developed hairy roots with the frequency ranging from 10% to 36%. Transformation efficiency was independent on the bacterial strain used. The results indicate that haploid sugar beet explants are amenable to transformation using A. rhizogenes, and that the efficiency of that process can be increased by applying short ultrasound treatment.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2014
• Tom: 61
• Numer: 1
• Opis fizyczny: p.13-17,fig.,ref.

Twórcy

autor

Klimek-Chodacka M.

• Institute of Plant Biology and Biotechnology, Faculty of Horticulture, University of Agriculture in Krakow, Krakow, Poland

autor

Baranski R.

• Institute of Plant Biology and Biotechnology, Faculty of Horticulture, University of Agriculture in Krakow, Krakow, Poland

Bibliografia

• Bessou C, Ferchaud F, Gabrielle B, Mary B (2011) Biofuels, greenhouse gases and climate change. A review. Agron Sustain Dev 31: 1-79.
• Biancardi EC, Larry GS, George NB, Marco D (2005) Genetics and breeding of sugar beet. Science Publishers, Enfield NH, USA.
• Bosemark NO (2007) Genetics and breeding. In Sugar beet. Draycott AP, ed, pp 50-88. Blackwell Publishing Ltd, Oxford, UK.
• Britton M, Escobar M, Dandekar A (2008) The oncogenes of Agrobacterium tumefaciens and Agrobacterium rhizogenes. In Agrobacterium: from biology to biotechnology. Tzfira T, Citovsky V, eds, pp 523-563, Springer New York. 
• Cai D, Thurau T, Tian Y, Lange T, Yeh KW, Jung C (2003) Sporamin-mediated resistance to beet cyst nematodes (Heterodera schachtii Schm.) is dependent on trypsin inhibitory activity in sugar beet (Beta vulgaris L.) hairy roots. Plant Mol Biol 51: 839-849. 
• Dillen K, Demont M, Tillie P, Rodriguez Cerezo E (2013) Bred for Europe but grown in America: the case of GM sugar beet. New Biotechnology 30: 131-135. 
• Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8: 377-424. 
• Gaba V, Kathiravan K, Amutha S, Singer S, Xiaodi X, Ananthakrishnan G (2006) The uses of ultrasound in plant tissue culture. In Plant Tissue Culture Engineering. Dutta Gupta S, Ibaraki Y, eds, pp 417-426, Springer Netherlands.
• Giri A, Narasu ML (2000) Transgenic hairy roots: recent trends and applications. Biotechnol Adv 18: 1-22. 
• Gurel C, Gurel S, Lemaux PG (2008) Biotechnology applications for sugar beet. CRC Crit Rev Plant Sci 27: 108-140.
• Hamill JD, Rounsley S, Spencer A, Todd G, Rhodes MJC (1991) The use of the polymerase chain reaction in plant transformation studies. Plant Cell Rep 10: 221-224.
• Haas JH, Moore LW, Ream W, Manulis S (1995) Universal PCR primers for detection of phytopathogenic Agrobacterium strains. Appl Environ Microbiol 61: 2879-2884. 
• Higgins JD, Newbury HJ, Barbara DJ, Muthumeenakshi S, Puddephat IJ (2006) The production of marker-free genetically engineered broccoli with sense and antisense ACC synthase 1 and ACC oxidases 1 and 2 to extend shelf-life. Mol Breed 17: 7-20.
• Hoshi Y, Kondo M, Mori S, Adachi Y, Nakano M, Kobayashi H (2004) Production of transgenic lily plants by Agrobacterium-mediated transformation. Plant Cell Rep 22: 359-364. 
• Jacq B, Lesobre O, Sangwan R, Sangwan-Norreel B (1993) Factors influencing T-DNA transfer in Agrobacterium-mediated transformation of sugarbeet. Plant Cell Rep 12: 621-624. 
• Jafari M, Norouzi P, Molboobi MA, Ghareyazie B, Valizadeh M, Mohammadi SA, Mousavi M (2009) Enhanced resistance to a lepidopteran pest in transgenic sugar beet expressing synthetic cry1Ab gene. Euphytica 165: 333-344.
• Jefferson R (1987) Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol Biol Rep 5: 387-405.
• Kishchenko EM, Komarnitskii IK, Kuchuk NV (2005) Production of transgenic sugarbeet (Beta vulgaris L.) plants resistant to phosphinothicin. Cell Biol Int 29: 15-19. 
• Klimek-Chodacka M, Baranski R (2013) Comparison of haploid and doubled haploid sugar beet clones in their ability to micropropagate and regenerate. Electron J Biotechnol 16: 1-1. Available from:  http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200001&lng=en. http://dx.doi.org/10.2225/vol16-issue2-fulltext-3.
• Lipp M, Bluth A, Eyquem F, Kruse L, Schimmel H, Van den Eede G, Anklam E (2001) Validation of a method based on polymerase chain reaction for the detection of genetically modified organisms in various processed foodstuffs. Eur Food Res Technol 212: 497-504.
• Liu Y, Yang H, Sakanishi A (2006) Ultrasound: Mechanical gene transfer into plant cells by sonoporation. Biotechnol Adv 24: 1-16. 
• McInnes E, Davey MR, Mulligan BJ, Davies K, Sargent AW, Morgan AJ (1989) Use of a disarmed Ri plasmid vector in analysis of transformed root induction. J Exp Bot 40: 1135-1144.
• Mishutkina IaV, Kamionskaia AM, Skriabin KG (2010) Generation of sugar beet transgenic plants expressing bar gene. Prikl Biokhim Mikrobiol 46: 89-95. 
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1: 473-497.
• Nehls R, Kraus J, Matzk A, Jansen R (2010) Transgenic varieties: Sugarbeet. Sugar Tech 12: 194-200.
• Ninković S, Djordjević T, Vinterhalter B, Uzelac B, Cingel A, Savić J, Radović S (2010) Embryogenic responses of Beta vulgaris L. callus induced from transgenic hairy roots. Plant Cell Tissue Organ Cult 103: 81-91.
• Offringa IA, Melchers LS, Regensburg-Tuink AJG, Costantino P, Schilperoort RA, Hooykaas PJJ (1986) Complementation of Agrobacterium tumefaciens tumor-inducing aux mutants by genes from the TR- region of the Ri plasmid of Agrobacterium rhizogenes. Proc Nat Acad Sci USA 83: 6935-6939.  
• Pathak M, Hamzah R (2008) An effective method of sonication-assisted Agrobacterium-mediated transformation of chickpeas. Plant Cell Tissue Organ Cult 93: 65-71.
• Rogers SO, Bendich AJ (1988) Extraction of DNA from plant tissues. Plant Mol Biol Manual A6: 1-10.
• Rudrappa T, Neelwarne B, Kumar V, Lakshmanan V (2005) Peroxidase production from hairy root cultures of red beet (Beta vulgaris). Electron J Biotechnol 8: 185-196.
• Smigocki A, Campbell L, Larson R, Wozniak C (2008) Sugar beet. In Compendium of Transgenic Crop Plants: Transgenic Sugar, Tuber and Fiber Crops. Kole C, Hall TC eds, pp 59-96. Blackwell Publishing, Oxford, UK.
• Taya M, Mine K, Kino-Oka M, Tone S, Ichi T (1992) Production and release of pigments by culture of transformed hairy root of red beet. J Ferment Bioeng 73: 31-36.
• Trick H, Finer J (1997) SAAT: sonication-assisted Agrobacterium-mediated transformation. Transgenic Res 6: 329-336.