Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2008 | 52 | 4 |
Tytuł artykułu

Involvement of progesterone, oxytocin, and noradrenaline in the molecular regulation of steroidogenesis in the corpus luteum of the cow

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of this study was to determine: (a) whether progesterone affects the expression of the gene for its own receptor, thereby affecting its own synthesis; (b) whether oxytocin (OT) and noradrenaline (NA) influence the expression of gene for receptor (R) of OT (OT-R), progesterone (PR-R), and β2-R, determined by means of real-time PCR; (c) whether OT, progesterone, and NA affects the protein level for receptors of OT and β2, determined by means of the Western blot. Luteal cells isolated from the corpus luteum (CL) on days 6-10 and 11-16 of the oestrous cycle were used in the study. The luteal cells were treated with luteinising hormone (LH; 100 ng/mL), progesterone (10⁻⁵ M), OT (10⁻⁷ M), NA (10⁻⁵ M), and prostaglandin (PG) E₂ (10⁻⁶ M) for 6 h for the gene expression and for 24 h for the measurement of receptor protein. Thereafter, medium was collected for the determination of progesterone concentration. LH and PGE₂ increased luteal secretion of P4 on days 6-10 and 11-16 after 6 h and 24 h stimulation. LH increased expression of the gene for PR-R on days 6-10 and 11-16 but PGE₂ only on days 6-10 of the oestrous cycle. OT stimulated gene expression for its own receptor on days 11-16, while progesterone only increased OT-R protein on days 6-10 of the cycle. The obtained data suggest a positive intra-ovarian feedback mechanism between progesterone and OT that enhances the function of CL in the cow.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
52
Numer
4
Opis fizyczny
p.573-580,fig.,ref.
Twórcy
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-718 Olsztyn - Kortowo, Poland
autor
  • Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-718 Olsztyn - Kortowo, Poland
Bibliografia
  • 1. Bloch K.: The biological synthesis of cholesterol. Science 1965, 150, 19-28.
  • 2. Bogacki M., Kotwica J.: Noradrenaline affects the post- translational synthesis of oxytocin in bovine corpus luteum. Theriogenology 1999, 52, 91-102.
  • 3. Bradford M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72, 248-254.
  • 4. Chomczynski P., Sacchi N.: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol- cloroform extraction. Anal Biochem 1987, 162, 156-159.
  • 5. Drummond A.E., Britt K.L., Dyson M., Jones M.E., Kerr J.B., O'Donnell L., Simpson E.R., Findlay J.K.:_Ovarian steroid receptors and their role in ovarian function. Mol Cell Endocrinol 2002, 191, 27-33.
  • 6. Ireland J.J., Murphee R.L., Coulson P.B.: Accuracy of predicting stages of bovine estrous cycle by gross appearance of the corpus luteum. J Dairy Sci 1980, 63, 155-160.
  • 7. Kalsner S.: Steroid potentiation of responses to sympathomimetic amines in aortic strips. Br J Pharmacol. 1969, 36, 582-593.
  • 8. Kotwica J., Bogacki M., Rękawiecki R.: Neural regulation of corpus luteum in cow. Dom Anim Endocrinol 2002, 23, 299-308.
  • 9. Kotwica J., Miszkiel G., Skarżyński D., Bogacki M.: Effect of progesterone on its own synthesis in bovine corpus luteum. Biol Reprod 1998, 58 (Suppl. 1), 338 [abstract].
  • 10. Kotwica J., Rękawiecki R., Duras M.: Stimulatory influence of progesterone on its own synthesis in bovine corpus luteum. Bull Inst Vet Pulawy 2004, 48, 139-145.
  • 11. Kotwica J., Skarżyński D., Jaroszewski J., Kotwica G.: The effect of noradrenaline on the release of progesterone and ovarian oxytocin in cattle: mechanism of action. Anim Reprod Sci 1991, 26, 179-191.
  • 12. Kotwica J., Skarżyński D., Młynarczuk J., Rękawiecki R.: Role of prostaglandin E₂ in basal and noradrenaline-induced progesterone secretion from corpus luteum in cow. Prostaglandins Other Lipid Mediat 2003, 70, 351- 359.
  • 13. Kotwica J., Skarżyński D.J., Jaroszewski J.J., Bogacki M.: Noradrenaline affects secretory function of corpus luteum independently on prostaglandins in conscious cattle. Prostaglandins 1994, 48, 1-10.
  • 14. Kotwica J., Skarżyński D.: Influence of oxytocin removal of corpus luteum on secretory function and duration of the estrus cyclein cattle. J Reprod Fert 1993, 97, 411-417.
  • 15. Lefkowitz R.J., Caron M.G. Molecular and regulatory properties of adrenergic receptors. Prog Horm Res 1987, 43, 469-497.
  • 16. Luck M.R., Payne J.H.: The neurocrine status of the mammalian ovary. Biol Reprod 1991, 57, B1-B8.
  • 17. Miszkiel G., Kotwica J.: Mechanism of action of noradrenaline on secretion of progesterone and oxytocin by the bovine corpus luteum in vitro. Acta vet Hung 2001, 49, 39-51.
  • 18. Miszkiel G., Skarżyński D., Bogacki M., Kotwica J.: Concentrations of catecholamines, ascorbic acid, progesterone and oxytocin in the corpora lutea of cyclic and pregnant cattle. Reprod Nutr Dev 1999, 39, 509-516.
  • 19. Miyamoto A., Schams D.: Oxytocin stimulates progesterone release from microdialyzed bovine corpus luteum in vitro. Biol Reprod 1991, 44, 1163-1170.
  • 20. Okuda K., Miyamoto A., Sauerwein H., Schweigert F.J., Schams D.: Evidence for oxytocin receptors in cultured bovine luteal cells. Biol Reprod 1992, 46, 1001-1006.
  • 21. Park O.K., Mayo K.E.: Transient expression of progesterone receptor messenger RNA in ovarian granulosa cells after the preovulatory luteinizing hormone surge. Mol Endocrinol 1991, 5, 967-978.
  • 22. Prakash B.S., Meyer H.H.D., Schallenberger E., Van de Wiel D.F.M.: Development of a sensitive enzymeimmunoassay (EIA) for progesterone determination in unextracted bovine plasma using the second antibody technique. J Steroid Biochem 1987, 28, 623-637.
  • 23. Rękawiecki R., Kotwica J.: Molecular regulation of progesterone synthesis in the bovine corpus luteum. Vet- Med Czech 2007, 52, 405-412.
  • 24. Rękawiecki R., Nowik M., Kotwica J.: Stimulatory effect of LH, PGE2 and progesterone on StAR protein, cytochrome P450 cholesterol side chain cleavage and 3β hydroxysteroid dehydrogenase gene expression in bovine luteal cells. Prostaglandins other Lipid Mediat 2005, 78, 169-184
  • 25. Sheldrick E.L., Flint A.P.: Transient uterine refractoriness after oxytocin administration in ewes. J Reprod Fertil 1986, 77, 523-529.
  • 26. Shroepfer G.J. Jr.: Sterol biosynthesis. Ann Rev Biochem 1982, 51, 555-585.
  • 27. Słomczyńska M., Krok M., Pierściński A.: Localization of the progesterone receptor in the porcine ovary. Acta Histochem 2000, 102, 183-191.
  • 28. Snook R.B., Brunner M.A., Saatman R.R., Hansel W.: The effect of antisera to bovine LH in hysterectomized and intact heifers. Biol Reprod 1969, 1, 49-58.
  • 29. Stefenson A., Owman C., Sjoberg N.O., Sporrong B., Walles B.: Comparative study of the autonomic innervation of the mammalian ovary, with particular regard to the follicular system. Cell Tissue Res 1984, 215, 262-266.
  • 30. Williams G.L.: Modulation of luteal activity in postpartum beef cows through changes in dietary lipid. J Anim Sci 1989, 67, 785-793.
  • 31. Wiltbank M.C., Gallagher K.P., Christensen A.K., Brabec R.K., Keyes P.L.: Physiological and immunocytochemical evidence for a new concept of blood flow regulation in the corpus luteum. Biol Reprod 1990, 42, 139-149.
  • 32. Yoshimoto Y., Sakumoto T., Arai R., Miyake A., Kimura H., Aono T., Tanizawa O., Maeda T.: Monoamine oxidase in rat ovary during the estrous cycle. A histochemical study by a new coupled peroxidatic oxidation method. Endocrinology 1986, 119, 1800-1804.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-713c39cb-94fe-4972-a37f-b760dba9a48a
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.