Differential expression of expressed sequence tags in alfalfa roots under aluminium stresse

• Autorzy: Fan F. , Li X.-W. , Wu Y.-M. , Xia Z.-S. , Li J.-J. , Zhu W. , Liu J.-X.
• Języki publikacji: EN

Abstrakty

EN

To gain a better understanding of differentially expressed sequence tags (ESTs) for aluminum (Al) tolerance and to investigate the molecular mechanisms of Al toxicity, cDNA subtraction libraries were generated from Al-stressed roots of alfalfa (Medicago sativa L.) compared with no Al-stressed ones, employing suppression subtractive hybridization. Using differential screening technique in which the probes were labeled with DIG, we identified 45 non-redundant ESTs in Al-stressed alfalfa root tips with significantly altered expression. Among the up-regulated ESTs, we have found genes encoding identified proteins, including malate dehydrogenase, 6-phosphogluconate dehydrogenase, peroxidase, and an ABC transporter, while the down-regulate genes included ATPase, secretory carrier membrane protein 2, pectinesterase inhibitor. In addition, two novel ESTs, EW678752 and EY976957, up- and down-regulated by Al stress were sequenced. Analyzed by real-time PCR, the expressions of EST EW678718, EW678739, EY976969 and EW678728, which encode for ABC transporter, malate dehydrogenase, peroxidase and 6-phosphogluconate dehydrogenase correspondingly, increased 1.64-, 2.75-, 3.27- and 6.54-folds, respectively, and the expression of EY976957 encoding for ATPase decreased 3.27 folds. The expression of EST EW678752 increased 34.54-fold, while that of EY976957 decreased 16.68 folds. It suggested that the two novel ESTs maybe play a significant role in the aluminum tolerance of alfalfa.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 2
• Opis fizyczny: p.539-546,fig.,ref.

Twórcy

autor

Fan F.

• College of Animal Sciences, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, No.268 Kaixuan Road, Hangzhou 310029, People's Republic of China

autor

Li X.-W.

• College of Animal Sciences, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, No.268 Kaixuan Road, Hangzhou 310029, People's Republic of China

autor

Wu Y.-M.

• College of Animal Sciences, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, No.268 Kaixuan Road, Hangzhou 310029, People's Republic of China

autor

Xia Z.-S.

• College of Animal Sciences, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, No.268 Kaixuan Road, Hangzhou 310029, People's Republic of China

autor

Li J.-J.

• College of Animal Sciences, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, No.268 Kaixuan Road, Hangzhou 310029, People's Republic of China
• Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, People's Republic of China

autor

Zhu W.

• College of Animal Sciences, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, No.268 Kaixuan Road, Hangzhou 310029, People's Republic of China

autor

Liu J.-X.

• College of Animal Sciences, Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, No.268 Kaixuan Road, Hangzhou 310029, People's Republic of China

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