Evaluation of the spatial arrangement of Purkinje cells in ataxic rat’s cerebellum after Sertoli cells transplantation

• Autorzy: Mohammadi R. , Heidari M.H. , Sadeghi Y. , Abdollahifar M.-A. , Aghaei A.
• Języki publikacji: EN

Abstrakty

EN

Background: Purkinje cells (PCs) pathology is important in cerebellar disorders like ataxia. The spatial arrangement of PCs after different treatments has not been studied extensively. Immunohistochemistry (IHC) analysis of cerebellum can give a proper tool for explaining the pathophysiology of PCs in ataxia. Here we stereologically analysed the 3-dimensional spatial arrangement of PCs in the cerebellum of rats after ataxia induction with 3-acetylpyridine (3-AP). Materials and methods: Ataxia was induced in rats by intraperitoneal injection of 3-AP (65 mg/kg). Spatial arrangement of PCs for differences in ataxic rats with (3-AP-SC) or without (3-AP) Sertoli cells (SCs) transplantation was evaluated using second-order stereology. The IHC method by using antibodies to anti-calbindin in the cerebellum was applied. Results: Our results showed that a random arrangement is at larger distances between PCs in 3-AP and 3-Ap-SC groups. Therefore the PCs are not normally arranged after 3-AP and SCs transplantation stored the spatial arrangements of the cells after ataxia induction in rats. IHC analyse shows that number of PCs was significantly improved after the SC transplantation. Conclusions: Segregation of PCs can be observed at some areas in the ataxic rats’ cerebellum. However, the spatial arrangement of PCs was unchanged in SCs transplanted rats. (Folia Morphol 2018; 77, 2: 194–200)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2018
• Tom: 77
• Numer: 2
• Opis fizyczny: p.194–200,fig.,ref.

Twórcy

autor

Mohammadi R.

• Department of Anatomy, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

autor

Heidari M.H.

• Department of Anatomy, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

autor

Sadeghi Y.

• Department of Anatomy, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
• Hearing Disorder Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran

autor

Abdollahifar M.-A.

• Department of Anatomy, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

autor

Aghaei A.

• Department of Anatomy, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Bibliografia

• 1. Akosman MS, Gocmen-Mas N, Karabekir HS. Estimation of Purkinje cell quantification and volumetry in the cerebellum using a stereological technique. Folia Morphol. 2011; 70(4): 240–244, indexed in Pubmed: 22117240.
• 2. Balaban C. Central neurotoxic effects of intraperitoneally administered 3-acetylpyridine, harmaline and niacinamide in Sprague-Dawley and Long-Evans rats: A critical review of central 3-acetylpyridine neurotoxicity. Brain Res Rev. 1985; 9(1): 21–42, doi: 10.1016/0165-0173(85)90017-7, indexed in Pubmed: 3158380.
• 3. Barker CF, Billingham RE. Immunologically privileged sites. Adv Immunol. 1977; 25: 1–54, doi: 10.1016/s0065-2776(08)60930-x, indexed in Pubmed: 345773.
• 4. Cendelin J. Transplantation and Stem Cell Therapy for Cerebellar Degenerations. Cerebellum. 2016; 15(1): 48–50, doi: 10.1007/s12311-015-0697-1, indexed in Pubmed: 26155762.
• 5. Chintawar S, Hourez R, Ravella A, et al. Grafting neural precursor cells promotes functional recovery in an SCA1 mouse model. J Neurosci. 2009; 29(42): 13126–13135, doi: 10.1523/JNEUROSCI.0647-09.2009, indexed in Pubmed: 19846700.
• 6. Dietrichs E. Clinical manifestation of focal cerebellar disease as related to the organization of neural pathways. Acta Neurol Scand Suppl. 2008; 188: 6–11, doi: 10.1111/j.1600-0404.2008.01025.x, indexed in Pubmed: 18439215.
• 7. Gasbarri A, Pompili A, Pacitti C, et al. Comparative effects of lesions to the ponto-cerebellar and olivo-cerebellar pathways on motor and spatial learning in the rat. Neuroscience. 2003; 116(4): 1131–1140, doi: 10.1016/s0306-4522(02)00780-7, indexed in Pubmed: 12617954.
• 8. Hemendinger R, Wang J, Malik S, et al. Sertoli cells improve survival of motor neurons in SOD1 transgenic mice, a model of amyotrophic lateral sclerosis. Exp Neurol. 2005; 196(2): 235–243, doi: 10.1016/j.expneurol.2005.07.025, indexed in Pubmed: 16242126.
• 9. Jiang YY, Cao BB, Wang XQ, et al. Cerebellar ataxia induced by 3-AP affects immunological function. Neuro Endocrinol Lett. 2015; 36(3): 246–256, indexed in Pubmed: 26313392.
• 10. Kemp KC, Cook AJ, Redondo J, et al. Purkinje cell injury, structural plasticity and fusion in patients with Friedreich’s ataxia. Acta Neuropathol Commun. 2016; 4(1): 53, doi: 10.1186/s40478-016-0326-3, indexed in Pubmed: 27215193.
• 11. Liu HW, Kuang YJ, Wu JC, et al. Intrastriatal transplantation of Sertoli cells may improve amphetamine-induced rotation and tyrosine hydroxylase immunoreactivity of the striatum in hemiparkinsonian rats. Brain Res. 1999; 838(1-2): 227–233, indexed in Pubmed: 10446339.
• 12. Ma W, Wang C, Su Y, et al. Expression of nerve growth factor and its receptor, tyrosine kinase receptor A, in rooster testes. Anim Reprod Sci. 2015; 161: 40–46, doi: 10.1016/j.anireprosci.2015.08.001, indexed in Pubmed: 26297622.
• 13. Maddocks S, Setchell BP. Recent evidence for immune privilege in the testis. J Reprod Immunol. 1990; 18(1): 9–18, doi: 10.1016/0165-0378(90)90021-w., indexed in Pubmed: 2213734.
• 14. Manto M, Haines D, Manto M. The cerebellum, cerebellar disorders, and cerebellar research--two centuries of discoveries. Cerebellum. 2008; 7(4): 505–516, doi: 10.1007/s12311-008-0063-7, indexed in Pubmed: 18855093.
• 15. Marmolino D, Manto M. Past, present and future therapeutics for cerebellar ataxias. Curr Neuropharmacol. 2010; 8(1): 41–61, doi: 10.2174/157015910790909476, indexed in Pubmed: 20808545.
• 16. Matsuura S, Shuvaev AN, Iizuka A, et al. Mesenchymal stem cells ameliorate cerebellar pathology in a mouse model of spinocerebellar ataxia type 1. Cerebellum. 2014; 13(3): 323–330, doi: 10.1007/s12311-013-0536-1, indexed in Pubmed: 24242763.
• 17. Mattfeldt T, Eckel S, Fleischer F, et al. Statistical analysis of reduced pair correlation functions of capillaries in the prostate gland. J Microsc. 2006; 223(Pt 2): 107–119, doi: 10.1111/j.1365-2818.2006.01604.x, indexed in Pubmed: 16911071.
• 18. Noorafshan A, Abdollahifar MA, Karbalay-Doust S. Stress changes the spatial arrangement of neurons and glial cells of medial prefrontal cortex and sertraline and curcumin prevent it. Psychiatry Investig. 2015; 12(1): 73–80, doi: 10.4306/pi.2015.12.1.73, indexed in Pubmed: 25670949.
• 19. Paxinos G, Watson C. The Rat Brain in Stereotaxic Coordinates: Hard Cover Edition. Academic Press. 2006: 451.
• 20. Rodriguez AI, Willing AE, Saporta S, et al. Effects of Sertoli cell transplants in a 3-nitropropionic acid model of early Huntington’s disease: a preliminary study. Neurotox Res. 2003; 5(6): 443–450, doi: 10.1007/bf03033174, indexed in Pubmed: 14715448.
• 21. Schmitz C, Grolms N, Hof PR, et al. Altered Spatial Arrangement of Layer V Pyramidal Cells in the Mouse Brain following Prenatal Low-dose X-Irradiation. A Stereological Study using a Novel Three-dimensional Analysis Method to Estimate the Nearest Neighbor Distance Distributions of Cells in Thick Sections. Cerebral Cortex. 2002; 12(9): 954–960, doi: 10.1093/cercor/12.9.954.
• 22. Weimann JM, Charlton CA, Brazelton TR, et al. Contribution of transplanted bone marrow cells to Purkinje neurons in human adult brains. Proc Natl Acad Sci U S A. 2003; 100(4): 2088–2093, doi: 10.1073/pnas.0337659100, indexed in Pubmed: 12576546.
• 23. Willing AE, Cameron DF, Sanberg PR. Sertoli cell transplants: their use in the treatment of neurodegenerative disease. Mol Med Today. 1998; 4(11): 471–477, doi: 10.1016/s1357-4310(98)01355-0, indexed in Pubmed: 9857366.

Identyfikatory

DOI

10.5603/FM.a2017.0091