Changes in the expression of galanin and galanin receptors in the wall of the colon in pigs experimentally infected with Brachyspira hyodysenteriae

• Autorzy: Wasowicz K. , Podlasz P. , Chmielewska M. , Losiewicz K. , Kaleczyc J. , Zmudzki J. , Zalecki M. , Pidsudko Z. , Lakomy M.
• Języki publikacji: EN

Abstrakty

EN

The expression of galanin (GAL) and its three receptors (GalR1, GalR2, and GalR3) were studied with real-time PCR in the colonic wall of pigs suffering from experimental colitis caused by the infection with Brachyspira hyodysenteriae. The expression was studied in the muscular membrane, mucosa/submucosa layer, and in lymphocytes isolated from mucosa/submucosa. The expression levels were normalized to glyceraldehyde-6-phosphate dehydrogenase (GAPDH) expression and compared to expression levels in control animals. GAL expression was found in all three studied compartments of the colonic wall. A significant decrease in GAL expression level was found in the mucosa/submucosa and in isolated lymphocytes, whereas the decrease was much less profound in the muscular membrane. In the case of galanin receptors their expression was found in all studied compartments of the colonic wall, however at different levels, as compared to GAPDH expression. The decrease of galanin receptors expression was found in all studied compartments of the colonic wall of the sick animals.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2014
• Tom: 58
• Numer: 1
• Opis fizyczny: p.23-28,fig.,ref.

Twórcy

autor

Wasowicz K.

• Department of Pathophysiology, Forensic Veterinary Medicine and Administration

autor

Podlasz P.

• Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

autor

Chmielewska M.

• Department of Pathophysiology, Forensic Veterinary Medicine and Administration

autor

Losiewicz K.

• Department of Pathophysiology, Forensic Veterinary Medicine and Administration

autor

Kaleczyc J.

• Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

autor

Zmudzki J.

• Department of Swine Diseases, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Zalecki M.

• Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

autor

Pidsudko Z.

• Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

autor

Lakomy M.

• Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

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