Somatic embryogenesis and plant regeneration from cell suspension culture of niger (Guizotia abyssinica Cass.)

• Autorzy: Naik P.M. , Murthy H. N.
• Języki publikacji: EN

Abstrakty

EN

Somatic embryogenesis was achieved from cell suspension cultures of niger (Guizotia abyssinica Cass.). Initially, friable embryogenic calluses were induced from cotyledonary leaves of niger on Murashige and Skoog (MS) agar medium containing 5 µM 2,4-Dichlorophenoxyacetic acid (2,4-D) and 0.5 µM kinetin (KIN). Cell suspension cultures were established by using embryogenic calluses in MS liquid medium containing 5 µM 2,4-D and 0.5 µM KIN. Initiation of somatic embryogenesis and development up to globular stage from embryogenic cell clumps occurred in the liquid medium itself. Thereafter embryogenic cell aggregates were transferred to MS agar medium supplemented with 3 µM KIN for embryo differentiation, whereas maturation of somatic embryos occurred in MS agar medium containing 10 µM abscisic acid.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 1
• Opis fizyczny: p.75-79,fig.,ref.

Twórcy

autor

Naik P.M.

• Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences

autor

Murthy H. N.

• Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences

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