Effect of long-term cultivation on resveratrol accumulation in a high-producin cell culture of Vitis amurensis

• Autorzy: Dubrovina A.S. , Kiselev K. V.
• Języki publikacji: EN

Abstrakty

EN

Resveratrol is a polyphenol, present in grapes, peanuts, and other plant sources, with a wide range of valuable biological activities. We established a Vitis amurensis cell culture accumulating high levels of resveratrol by introducing the rolB gene of Agrobacterium rhizogenes in the V. amurensis genome, and studied the stability of resveratrol accumulation during 27 months of continuous subculturing. This study demonstrates a decline in the high level of resveratrol production by the rolB transgenic cell line during its long-term cultivation. Elicitation of the rolB transgenic calli with methyl jasmonate and salicylic acid, which are known to stimulate the production of plant secondary metabolites, resulted in a recovery of resveratrol accumulation in the rolB transgenic cell culture, while the empty vector-transformed culture with trace starting content of resveratrol exhibited low inducibility to the treatment.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 3
• Opis fizyczny: p.1101-1106,fig.,ref.

Twórcy

autor

Dubrovina A.S.

• Laboratory of Biotechnology, Institute of Biology and Soil Sciences, Far Eastern Branch of Russian Academy of Sciences, 690022 Vladivostok, Russia

autor

Kiselev K. V.

• Laboratory of Biotechnology, Institute of Biology and Soil Sciences, Far Eastern Branch of Russian Academy of Sciences, 690022 Vladivostok, Russia

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