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The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.
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p.1-8,fig.,ref.
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autor
- Division of Cytogenetic and Plant Breeding, Department of Botany, Annamalai University, Annamalai Nagar 608002, India
autor
- Division of Cytogenetic and Plant Breeding, Department of Botany, Annamalai University, Annamalai Nagar 608002, India
autor
- Division of Cytogenetic and Plant Breeding, Department of Botany, Annamalai University, Annamalai Nagar 608002, India
autor
- Division of Cytogenetic and Plant Breeding, Department of Botany, Annamalai University, Annamalai Nagar 608002, India
Bibliografia
- [1] Arulbalachandran D, 2006. Effect of physical and chemical mutagenesis in black gram (Vigna mungo (L.) Hepper). Ph.D. Thesis, Faculty of Science, Annamalai University, Annamalai Nagar, India.
- [2] Ashok Kumar G., V. Ponnuswami, Research Journal of Agricultural Sciences 1(3) (2010) 193-195.
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- [4] Basu S. K., A. K. De, 2003. Capsicum: Historical and Botanical Perspectives. Citation information Capsicum. The genus Capsicum Print ISBN: 978-0-415- 29991-6, eBook ISBN: 978-0-203-38115.
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- [8] Naugžemys D., S. Zilinskaite, J. Denkovskij, J. Patamsytė, J. Literskis, D. Zvingila, Biologia 53(3) (2007) 34-39.
- [9] Salvador M. H., Genetic resources of chilli (Capsicum spp.) in Mexico. Proc. of the 16th Int. Pepper Conf., Tampico, Tamaulipas, Mexico, November, 2002, pp. 10-12.
- [10] Senthamizh Selvi B., V. Ponnuswami, P. S. Kavitha, Adv. Nat. Appl. Sci. 2(3) (2008) 129-134.
- [11] Subramanian V., Gurtu S., Nageswara Rao, Nigam S. N., Genome 43 (2000) 656-660.
- [12] Wachira F. N., Waugh R., Hackett C. A., Powell W., Genome 38 (1995) 201-210.
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- [14] Yüzbasıoğlu E., S. Özcan, L. Açık, Genet. Resour. Crop Ev. 53 (2006) 507-514.
- [15] E. Sanjai Gandhi, A. Sri Devi, International Letters of Natural Sciences 5 (2014) 18-23.
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Bibliografia
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