The detection of enteroviruses in sewage using Caco-2 cells

• Autorzy: Wieczorek M. , Kuryk L. , Witek A. , Diuwe A. , Litwinska B.
• Języki publikacji: EN

Abstrakty

EN

The work presented here demonstrates the utility of Caco-2 cells to detect enteroviruses in sewage. Viruses were concentrated by beef extract elution and organic flocculation prior to analysis by cell culture assays and RT-PCR. Enteroviruses were detected in all sewage samples, but only one sample was positive solely in RT-PCR assay. We proved that Caco-2 cells were more effective than RD and L20B cells in enterovirus isolation, depending on procedures used in the inoculation process.

Słowa kluczowe

EN

detection; enterovirus; sewage; Caco-2 cell

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2013
• Tom: 62
• Numer: 1
• Opis fizyczny: p.97-100,ref.

Twórcy

autor

Wieczorek M.

• Department of Virology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland

autor

Kuryk L.

• Department of Virology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland

autor

Witek A.

• Department of Virology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland

autor

Diuwe A.

• Department of Virology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland

autor

Litwinska B.

• Department of Virology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland

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